肠道上皮细胞损伤与Th1分泌干扰素γ关系的研究

 目的 探讨辅助性T细胞在肠黏膜损伤中的作用.方法 大鼠上皮细胞系(IEC-6)用刮匙轻刮IEC-6单细胞层产生"十"字缺损区,分别将大鼠重组IFN-γ(5 ng/ml)和IL-4(5 ng/ml)加入到细胞培养液中,培养IEC-6细胞16 h,观察细胞缺损区的面积,评价不同因子对细胞损伤后修复的影响.结果 IFN-γ抑制EC-6细胞损伤后的修复(P＜0.05);在有或无H2O2 0.25 mM氧化应激的情况下,IL-4 可以促进IEC-6 细胞损伤后的修复,但与对照组相比差异无统计学意义(P＞0.05).结论 Th1细胞可能通过分泌INF-γ调节IEC-6细胞损伤后的修复.

Intestinal epithelial injury is related to induction of Th1 cell-derived interferon gamma

Objective To investigate the effects of Thl during intestinal epithelial injury. Methods A denuded epithelial ＂crosscenter＂ wound was created in a standardized fashion by scraping the IEC - 6 monolayers with a gel loading pipet tip. Rat epithelial cell line （IEC - 6 cells ） was treated with IFN -γ（5ng/ml） or IL - 4（ 5ng/ml ）. The denuded wound area （ROI） was quantified. Wound repair was calculated as the reciprocal of denuded wound ROI. Result IEC - 6 cells treated with IFN - γ inhibited wound repair（ P 〈 0.05）, in contrast, IL - 4 promoted IEC - 6 cell wound repair. Conclusions IFN -γ is involved in inhibition of epithelial repair in IEC - 6 cells. Thl regulates epithelial repair of IEC - 6 cells by secreting.