溴氰菊酯对染毒孕鼠仔鼠神经行为发育的影响及中药干预作用

目的:观察溴氰菊酯(DM)雌性大鼠孕期染毒对子代神经行为发育的影响及补肾健脾复方助孕3号对DM发育毒性的干预作用。方法:将孕鼠分为模型组、中药组、牛磺酸组和空白对照组。除空白对照组外,其他3组自妊娠第1～15天经口灌服1/20LD50DM(6.93mg/kg),另灌服生理盐水、成人等效剂量中药液及牛磺酸溶液(0.2g/kg)。待孕鼠分娩后观察仔鼠断乳前神经行为发育指标及断乳后行为。结果:①模型组仔鼠前肢定位反射、平面翻正反射、空中翻正反射达标时间分别为(7.00±0.00)天、(6.20±1.64)天和(13.70±0.47)天。各组仔鼠的达标时间均在正常范围内。②避暗实验的结果显示各组仔鼠在记忆获得、记忆巩固、记忆再现方面差异无统计学意义。模型组在训练时、24h后、7天后的电击次数分别是(1.08±0.79)、(0.17±0.39)和(0.25±0.45)。结论:具有神经毒性时DM似乎并未对仔鼠的神经系统发育产生不良影响,补肾健脾中药对仔鼠神经系统发育干预作用不明显。

Effect of deltamethrin on neurobehavioral development of offsprings of intoxicated rats and intervention of Chinese herbal medicine

Objective:To observe the effect of deltamethrin on neurobehavioral development of offsprings of female intoxicated rats in pregnancy and intervention of Chinese herbal medicine on developmental toxicity of deltamethrin.Methods:The pregnant rats were divided into model group,Chinese medicine group,taurine group and blank control group.Except blank control group,the rats in the other three groups were orally fed 1/20 LD 50 deltamethrin (6.93 mg/kg),then they were orally fed normal saline,adult dosage of Chinese herbal medicine and taurine solution (0.2 g/kg),respectively.After delivery,the indexes of neurobehavioral development of offsprings before ablactation and behaviors after ablactation were observed.Results:The appearing times of placing reflex,surface righting reflex and air righting reflex of anterior limbs in model group were (7.00±0.00) days,(6.20±1.64) days and (13.70±0.47) days,respectively,the appearing times in the four groups were within normal range.The results of passive avoidance test revealed that there was no significant difference in memory acquisition,consolidation and reappearance among the four groups,the number of electric shocks during training,24 hours later and 7 days later were (1.08±0.79),(0.17±0.39) and (0.25±0.45) times,respectively.Conclusion:Exposure to deltamethrin in pregnant rats doesn't affect the neurobehavioral development of offsprings,the intervention of Chinese herbal medicine is not obvious.