米非司酮对绒毛组织中MTA3、ER及PR的影响

目的:了解米非司酮对绒毛组织中MTA3(Metastasis-associatedgene3)的表达及对雌、孕激素受体的影响。方法:对120例停经49天以内临床证实为早期妊娠,要求终止妊娠的妇女,根据停经天数的不同分为停经45天以下及45～49天两大组,每组60例,各组随机分为3小组,分别予米非司酮100mg,200mg,以及对照组。应用RT-PCR法检测各组MTA3的表达。并从各组中各自随机挑选5例,用ERα、PR单克隆抗体免疫组织化学法检测绒毛组织的ERα、PR水平。结果:4组用米非司酮的早孕妇女绒毛中MTA3表达均较对照组增高,差异有统计学意义(P<0.05),并表现为剂量相关性,随着米非司酮的剂量增加,MTA3的表达增高。服药组ERα水平增高,阳性物质定位于胞浆中,胞核中阴性。服药组PR水平低于对照组。结论:米非司酮用于早孕妇女可能通过直接或间接的途径影响雌激素通路,导致滋养细胞m-RNA水平MTA3表达增多,从而有可能使一些细胞黏附分子丢失而导致滋养细胞的侵袭能力的改变,达到抗早孕目的。

Effects of mifepristone on MTA3, ER and PR in the trophoblast cells of human villus

Objective:To study the effects of mifepristone on metastasis-associated gene 3(MTA3),estrogen receptors(ER) and progestin receptors(PR) in the trophoblast cells of human villus.Methods:120 early pregnant women,who terminated their pregnancy voluntarily,were divided into two teams:pregnancy within 45 days and pregnancy within 45～49 days.Each team was divided into 3 groups randomly:women in Group 1 were given mifepristone 25 mg twice a day for 2 days;while women in Group 2 were given 100 mg once a day for 2 days;Group 3 was control group.The expression of MTA3 in each group was detected.30 women were obtained for immunohistochemical ERα and PR analysis by monoclonal antibody.Results:Mifepristone increased the expression of MTA3(P<0.05),which was related to doses.The expression of ERα in treatment group was higher than that in control group,while the expression of PR in treatment group was lower than that in control group.Conclusion:Mifepristone may influence ER pathway directly or indirectly,increase the expression of MTA3 on mRNA level.