热诱导人胶质瘤U251细胞凋亡作用

目的 观察不同加热温度及不同加热时间对人胶质瘤U251细胞凋亡的影响.方法 采用恒温加热(43、44、45℃各1、2、3、4 h)处理细胞,后继续培养12 h.应用Annexin V-FITC/PI双染、瑞氏-姬姆萨染色、PI单染检测细胞凋亡率、凋亡细胞形态学、细胞周期生化学特征的变化情况.结果 随温度升高和诱导时间延长U251细胞凋亡率增加,44℃3 h凋亡率最高达40.10%(P<0.01);光镜下可见凋亡细胞出现膜小泡,凋亡小体;PI单染有凋亡峰及细胞周期改变.结论 44℃热诱导3 h是促使U251细胞凋亡的最佳条件,热诱导凋亡细胞具有典型的形态、生化学特征变化.

The effect of hyperthermia induce on U251 glioma cells apoptosis

Objective To study the effect of different temprature and time points on human glio-ma U251 cells apoptosis induced by hyperthermia. Methods Human U251 glioma cells were cultured in vitro and induced by hyperthermia 43,44,45℃ for 1,2,3,4 hours respectively and then routine culture for 12 hours. The apoptotic cell morphology was observed by Wright-Giemsa staining, the percentage of the cell apoptosis and the alteration of cell cycle were detected by FCM throgh Annexin V-FITC and PI stai-ning. Results The percentage of the cell apoptosis increased with hightening tempreture and prolonging time and the maximum rate of apoptosis was 40.10% at 44 centi-degree for 3 hours. Typical apoptotic bodies or membranosomas appeard. Apoptotic peak and the change of cell cycle were tested by FCM. Con-clusion 3 hours moderate hyperthermia at 43 centi-degree is the effective heat-dosage to induce human glioma U251 cells and the heat-induced apoptotic cells have typical morphology and biochemics character change.