喉癌组织及手术切缘Survivin、COX-2表达与局部复发的关系

目的检测喉癌组织及手术切缘中Survivin、COX-2表达情况，探讨两者的表达与术后局部复发的关系。方法选择62例原发灶为鳞状细胞癌、手术切缘组织病理学肿瘤阴性者，对喉癌组织及切缘连续切片，行HE染色，以免疫组化法分别进行Survivin及COX-2检测。结果62例喉癌患者原发灶组织中Survivin（＋）48例及COX-2（＋）38例的复发率分别为33．3％（16／48）和26．3％（10／38）高于阴性者14．3％（2／14）和12．5％（3／24），差异有统计学意义（P〈0．05）；手术切缘中Survivin（＋）27例及COX-2（＋）19例的复发率分别为29．6％（5／27）和26．3％（5／19）高于阴性者11．4％（3／35）和4．7％（2／43），差异有统计学意义（P〈0．05）。喉癌组织中Survivin及COX-2表达同时异常，且手术切缘组织Survivin及COX-2表达也同时异常，术后复发率为100％（5／5）。结论Survivin及COX-2检测可以作为预测喉癌预后的生物学标志，在病理学阴性手术切缘组织中两者联合检测对于判断术后局部复发有更大的意义。

Relationship of local recurrence with expression of protein Survivin and COX-2 in laryngeal carcinoma and its surgical margins

Objective To explore the relationship of the local recurrence with the expression of protein Survivin and COX-2 in the primary lesions and the surgical margins of laryngeal carcinoma.Methods The primary lesions and the surgical margins of laryngeal carcinoma of 62 patients were made into serial sections. Immunochemical method (HE staining) was used to detect the expression of Survivin and COX-2 protein.Results In these 62 cases of patients, the recurrent rates of the primary lesions positive for Survivin(48 cases) and COX-2(38 cases) were 33.3% (16/48) and 26.3%(10/38)respectively, which were significantly higher than those negative for them14.3% (2/14) and 12.5%(3/24)(P<0.05);the recurrent rates of surgical margins positive for Survivin (27 cases) and COX-2(19 cases) were 29.6% (5/27) and 26.3% (5/19) respectively, which were significantly higher than those negative for them11.4%(3/35) and 4.7%(2/43)(P<0.05). The expression of Survivin and COX-2 proteins in the primary lesions and surgical margins was abnormal; the recurrent rate of the laryngeal carcinoma was 100% (5/5) after surgery.Conclusion Survivin and COX-2 protein can be used as biomarkers for local recurrence of laryngeal carcinoma after operation. Detection of Survivin and COX-2 in both the primary lesions and the surgical margins may be of greater significance in forecasting local recurrence.