| 多色FISH技术快速检测未培养羊水细胞染色体 |
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| 引用本文： | 代作林,张启先,丁显平.多色FISH技术快速检测未培养羊水细胞染色体[J].中国优生与遗传杂志,2010(4):48-49,F0004. |
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| 作者姓名： | 代作林 张启先 丁显平 |
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| 作者单位： | 四川省大英县人民医院;四川大学生命科学学院遗传医学研究所; |
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| 摘 要： | 目的探讨采用多色荧光原位杂交（fluo rescence in situ hybridization,FISH）技术检测未培养羊水细胞染色体异常的可行性。方法应用着丝粒探针、特异性序列探针对16例孕妇的未培养羊水细胞进行FISH分析,对5条染色体（13号、18号、21号、X和Y）进行检测并和常规核型分析进行对照。结果16例羊水细胞检测均获得诊断结果,发现1例异常胎儿为18-三体。FISH检测与常规核型分析结果一致。结论多色FISH技术快速、简便、可靠,可一次实验检测多条染色体异常。具有较大临床应用价值。
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| 关 键 词： | 荧光原位杂交 羊水细胞 染色体 |
Rapid detection of chromosome in uncultured amniotic fluid by multi-fluorescence in situ hybridization |
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| DAI Zuo-lin,ZHANG Qi-xian,DING Xian-ping..Rapid detection of chromosome in uncultured amniotic fluid by multi-fluorescence in situ hybridization[J].Chinese Journal of Birth Health & Heredity,2010(4):48-49,F0004. |
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| Authors: | DAI Zuo-lin ZHANG Qi-xian DING Xian-ping |
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| Institution: | DAI Zuo-lin1,ZHANG Qi-xian2,DING Xian-ping2.(1.People\'s Hospital of Daying County,Daying Sichuan 629300,2.Institute of Medical Genetics,College of Life Science,Sichuan University,Chengdu 610064) |
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| Abstract: | Objective:To study the feasibility of using multi-fluorescence in situ hybridization(FISH) to detect numeral abnormalities of chromosome of Uncultured Amniotic Fluid.Methods:16 cases were detected by 13,18,21,X and Y chromosome FISH probes,and the results were compared with karyotype analysis ones.Results:one case was trisome 18 and the results of two methods was proved coincide.Conclusion:Multi-FISH is rapid,simple and reliable.furthermore,chromosomes can be detected simultaneously.Thereby,FISH is valuable... |
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| Keywords: | FISH Interphase amniocyte cell Chromosomes |
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