| 阿司匹林对结肠癌细胞凋亡及环加氧酶-2基因表达的影响 |
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| 引用本文： | 艾志龙,陆维祺,邵叶波,沈兆忠. 阿司匹林对结肠癌细胞凋亡及环加氧酶-2基因表达的影响[J]. 中国临床医学, 2004, 11(5): 770-772 |
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| 作者姓名： | 艾志龙 陆维祺 邵叶波 沈兆忠 |
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| 作者单位： | 1. 复旦大学附属中山医院外科,上海,200032
2. 复旦大学附属肿瘤医院分子生物学实验室,上海,200032 |
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| 基金项目： | 上海市科技发展基金项目 (编号 :0 0 41190 5 4) |
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| 摘 要： | 目的：探讨阿司匹林对结肠癌化学预防的机理。方法：培养结肠癌细胞株Caco-2，用不同浓度阿司匹林进行干预，对细胞增殖、凋亡及环加氧酶-2(COX-2)基因表达进行观察、检测。结果：高浓度阿司匹林组细胞大量死亡．低浓度促进细胞凋亡；与对照组相比，随着阿司匹林浓度增加，COX-2基因表达逐渐减少。结论：低浓度阿司匹林能促进结肠癌细胞凋亡，下调COX-2基因表达。
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| 关 键 词： | 阿司匹林 凋亡 基因表达 结肠癌细胞 环加氧酶-2 COX-2 癌化 下调 增加 促进 |
The Influence of Aspirin to Apoptosis and COX-2 Expression of Caco-2 Cell |
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| Ai Zhilong Lu Weiqi Shao Yebo,et al.. The Influence of Aspirin to Apoptosis and COX-2 Expression of Caco-2 Cell[J]. Chinese Journal Of Clinical Medicine, 2004, 11(5): 770-772 |
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| Authors: | Ai Zhilong Lu Weiqi Shao Yebo et al. |
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| Abstract: | Objective: To study the mechanism of chemoprevention of colorectal cancer by aspirin through observing its effect on colonic cell line. Methods: Different concentrations of aspirin were used in the culture of colonic cell-Caco-2, its proliferation and apoptosis were observed during the culture and the expression of COX-2 gene was assayed by RT-PCR. Results: Most cells died in the culture medium with high concentration aspirin, but the lower concentration of aspirin promoted. The COX-2 gene expression decreased with the increasing of the concentration of aspirin. Conclusion:The lower concentration of aspirin could promote apoptosis of colonic cell,down-regulate the expression of COX-2. |
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| Keywords: | Aspirin Colonic cancer cell Apoptosis Cyclooxygenase-2 |
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