多重聚合酶链反应检测食品中致病性小肠结肠炎耶尔森菌和伤寒沙门氏菌

目的 ]快速准确地检测致病性小肠结肠炎耶尔森菌和伤寒沙门氏菌 .方法 ]根据小肠结肠炎耶尔森菌的ail基因和ST的H1 d基因顺序设计二对引物 ,通过聚合酶链反应检测食品中的小肠结肠炎耶尔森菌和伤寒沙门氏菌 ,扩增产物经电泳 ,分别出现与已知阳性对照细菌条带大小一致的 170和 4 5 8bp的ail和H1 d基因片断的条带时即判定该样品为阳性 .结果和结论 ]本法特异性强、灵敏度高 ,能在60h内出结果 ,适用于食品中小肠结肠炎耶尔森菌和伤寒沙门氏菌的快速检验 .

Detection of pathogenic Yersinia enterocolitica and Salmonella typhi by multiplex PCR in food

OBJECTIVE?To explore a rapid, economical and sensitive procedures for the detection of Yersinia enterocolitica and Salmonella typhi. ?METHODS?Using multiplex PCR, the sequence of the ail gene of Y. enterocolitica and the H1-d gene of S. typhi were detected. The PCR amplified 170 and 458 bp fragments of the ail and H1-d gene, respectively. By the DNA marker or known size fragments, the bacteria were determined if the same size bands were found via electrophoresis. ?RESULTS AND CONCLUSION?The method is not only highly specific and sensitive but also capable of providing a clear cut result within 60 hours that a sample was submitted. It is capable of detecting Yersinia enterocolitica and Salmonella typhi simultaneously in food.