CA-1对Baegle犬的毒性试验研究

目的 :观察经口给予CA - 1对Baegle犬所产生的毒性反应、中毒症状、毒作用靶器官和无毒性反应剂量 ,为CA - 1的开发研究提供毒理学依据 .方法 :CA - 1以 1 0、 3 0、 6 0 g/kg体重连续 90d经口给予Baegle犬 ,每周 6d ,停药恢复 30d的亚慢性毒性试验 .结果 :给药期和停药恢复期动物一般状况良好 ,体重增长 ,未见中毒反应 .骨髓象检查发现高剂量组晚幼粒细胞和浆细胞数均高于其他三个组 ,有显著性差异(P <0 0 5 ,P <0 0 1) .血液学、血清生化、心电图及病理组织学检查均未见由CA - 1所致的异常改变 .结论 :试验结果提示经口给予CA - 190d对Beagle犬未产生毒性反应 ,该试验结果可作为CA - 1新药开发研究的安全性参考依据 .

Toxicological Evaluation of CA-1 in Beagle Dogs

Objective: CA-1 is an extract from a herb , cyanotis arachnoidea. The study aims to identify the toxicity effects of CA-1 on target organs and the safety margins of CA-1. Methods: CA-1 1.0, 3.0, 6.0g/kg body weight were given orally for 90 days, and after 30 days of ceasing treatment, subchronic studies were conducted in Beagle dogs of both genders. Results: There was no toxic reaction, and body weight, blood parameters, electrocardiogram, organ weight ratio and histopathological findings were similar in control and treated groups. Conclusion: The subchronic studies support a wide safety margin of CA-1.The preclinical toxicogical evaluation of CA-1 is a useful reference for novel drug development of CA-1.