复合膜异位诱导成骨研究

目的将基因重组人骨形态发生蛋白-2（rhBMP-2）与胶原、聚乳酸/聚羟基乙酸共聚体（PLGA）复合，制成rhBMP-2/Co/PLGA复合膜，观察其异位诱导成骨情况，评价其生物特性。方法取雄性昆明小鼠48只，随机分成Co/PLGA复合膜组与rhBMP-2/Co/PLGA复合膜组。在小鼠右后肢大腿肌间隙内分别植入2种复合膜材料。术后7、14、28 d，各组分别处死8只动物，标本行肉眼、软X线照相和组织学观察。结果2组动物术后情况良好。Co/PLGA复合膜组术后7 d复合膜开始溶解，14 d膜结构不连续，28 d大部分溶解；软X线和组织学观察未见异位骨生成。rhBMP-2/Co/PLGA复合膜组软X线和组织学观察均有骨样结构形成，与Co/PLGA复合膜组相比复合膜降解速度缓慢。结论Co/PLGA是rhBMP-2的良好载体，参与rhBMP-2诱导成骨活性的表达，使其在局部发挥更持久的生物活性。

Heterotopic complex membrane induces bone formation

Objective The composite membrane was made by recombinant human bone morphogenetic protein-2 （rhBMP-2）, collagen（Co）, polylactic-co-glycolic acid（PLGA）. To observe the ectopic bone formation and evaluate the capability of rhBMP-2/Co/PLGA complex membrane. Methods 48 male Kunming adult mice were divided into two groups randomly: Co/PLGA complex membrane group and rhBMP -2/Co/PLGA complex membrane group. After etherized, an incision was made on the outer muscle and two groups of complex membranes were implanted into the right side hind limb muscle pouch. 8 mice from each group were sacrificed at 7, 14, 28 d. The hind limbs were removed, examined by soft X-ray, HE staining and light microscope examination. Results Wounds healed well, no infections and rejections were observed. In the Co/PLGA group, partial implanted membrane degradated and absorbed at 7 d. The membrane collagen fiber appeared loose at 14 d. The membrane lost its intact to disrupted at 28 d. No ectopic bone formation was found. In the rhBMP-2/Co/PLGA group, ectopic bone formation was found by eye, soft Xray and histological examinations. The rhBMP-2/Co/PLGA complex membrane degraded slower than Co/PLGA complex membrane. Conclusion Co/PLGA can provide a carrier for rhBMP-2 to have stronger effect of biological activity.