神经生长因子对人血管内皮细胞增殖作用的研究

目的:研究神经生长因子(Nerve growth factor,NGF)对人血管内皮细胞(Human vascular endothelial cell,HEVC)增殖作用的影响。方法:将NGF作用于体外培养的HEVC304,利用酶联免疫反应检测其增殖率,利用免疫组织化学的方法检测对增殖细胞核抗原(Proliferating cell nuclear antigen,PCNA)的表达和标记指数。结果:NGF作用组较对照组其细胞增殖率明显增加(P<0.001),NGF中和抗体组其细胞增殖率下降明显(P<0.05)。NGF作用组较对照组能明显增加HEVC对PCNA的表达和PCNA标记指数(P<0.05),NGF中和抗体组其PCNA表达和PCNA标记指数明显减少(P<0.05)。结论:NGF能明显促进HEVC的增殖。

Study of effect of nerve growth factor on the human vascular endothelial cell proliferation

Objective To investigate the effect of nerve growth factor(NGF) on human vascular endothelial cell. Methods The effect of NGF on human umbilica vein vascular endothelial cell were used in the third passage. Endothelial cell proliferative rate was assessed using the enzyme linked immunoassay. The proliferating cell nuclear antigen expression and proliferating cell nuclear antigen lable index were assessed using the immunnohis-chemistry. Results Compared with the control group, the NGF groups whose endothelial cell proliferative rate were increased significantly( P<0. 001) NGF neutralizing antibody de creased the mitogenic effect of NGF significantly( P< 0.05) .NGF also increased PCNA expression and PCNA lable index on HEVC304 . NGF-neutralizing antibody de-creaed PCNA expression on NGF-stimulated HEVC304( P<0. 05). Conclusion NGF could significantly induced the mitosis of human umbilica vein vascular endothelial cell.