胃癌组织中H.pylori感染与bFGF蛋白表达关系及其临床意义

目的 :探讨幽门螺旋杆菌感染(Helicobacterpylori,H .pylori)与碱性成纤维细胞生长因子 (basicfibroblastgrowthfac tor ,bFGF)蛋白表达的关系及其与胃癌发生和发展的关系。方法 :应用免疫组化方法检测了 63例胃癌存档标本和 10例正常胃组织中bFGF的蛋白表达。结果 :63例胃癌存档蜡块中有 45例阳性 ,Hp阳性胃癌组和转移性胃癌组显著高于Hp阴性胃癌组和非转移性胃癌组 ,P <0 0 1。阳性着色定位于肿瘤细胞、血管内皮细胞及肿瘤基质。结论 :H .pylori感染增强了bFGF的表达 ,bFGF可能通过自分泌和旁分泌机制诱导胃癌血管生成 ,或通过胞内分泌作用刺激某些酶的产生 ,从而促进肿瘤的浸润。

Clinical significance and relationship between Helicobacter pylori infection and expression of basic fibroblast growth factor in gastric cancer

OBJECTIVE: To study the relationship between Helicobacter pylori (H.pylori) infection and the expression of basic fibroblast growth factor (bFGF) in gastric cancer and to explore their significance in gastric cancer. METHODS:The expression of bFGF protein of 63 samples of gastric cancer kept in archives were detected by using the immunohistochemical assay with a monoclonal antibody against bFGF. RESULTS:There was no positive signal in 10 normal gastric mucosas. bFGF protein was localized in tumor cells and endothelial cells. Forty-five of 63 samples in archive was positive for bFGF protein. There a was significant difference between Helicobacter pylori positive and negative cancers (P<0.01) and the difference was also found between metastasis and non-metastasis gastric cancers. CONCLUSIONS: H.pylori infection reinforces the expression of bFGF. bFGF may induce the angiogenesis of gastric cancer by autocrine and paracrine pathways,or promote tumor invasion by stimulating some enzymes production possibly by an intracrine pathway.