大黄虫丸拆方对动脉粥样硬化家兔血管平滑肌细胞增殖与凋亡的影响

目的研究大黄庶虫]虫丸拆方对动脉粥样硬化（AS）模型家兔胸主动脉血管平滑肌细胞（VSMC）增殖与凋亡的影响。方法采用免疫性内皮损伤合并高脂饲料喂养的方法建立家兔AS模型，通过SP免疫组化法检测增殖细胞核抗原（PCNA）在VSMC中的表达，缺口末端标记法（TUNEL）观察VSMC的凋亡情况。结果与模型对照组比较，大黄庶虫]虫丸拆方各功效组分均可使AS模型家兔胸主动脉VSMC中PCNA阳性细胞显著减少（P〈0．01），而凋亡阳性细胞数显著增加（P〈0．01）。结论大黄庶虫]虫丸各功效组分通过抑制VSMC增殖、诱导其凋亡，从而调节VSMC增殖和凋亡之间的平衡，发挥抗AS作用，其中拆方1号是主要组分。

Effect of the Disassembled Recipes of Dahuang Zhechong Pill on Proliferation and Apoptosis of Vascular Smooth Muscle Cells in Atherosclerotic Rabbits

OBJECTIVE: To study the effects of the disassembled recipes of Dahuang Zhechong Pill (DRDZP) on proliferation and apoptosis of vascular smooth muscle cell (VSMC) of thoracic aorta in atherosclerotic rabbits. METHODS: The atherosclerotic rabbit model was induced by high-cholesterol diet and immune injury of endothelium. Expression of proliferating cell nuclear antigen (PCNA) in VSMC was detected by SP immunohistochemical technique, and VSMC apoptosis was observed with TUNEL technique. RESULTS: Compared with the model control, PCNA expression in VSMC decreased, while apoptotic cells increased significantly in all the groups treated by DR-DZP (P < 0.01). CONCLUSION: All the DR-DZP could inhibit VSMC proliferation and promote its apoptosis to modulate the balance between cell proliferation and apoptosis, so as to exert antiatherosclerotic action, among which the disassembled-recipe I is the main composition to contribute to antiatherosclerotic action of Dahuang Zhechong Pill.