莫达非尼抑制过氧化氢诱导的PC12细胞凋亡

目的:研究莫达非尼(modafinil)对过氧化氢诱导的PC12细胞凋亡的抑制作用,并对其机制进行探讨.方法:以噻唑兰(MTT)法测定PC12细胞存活率;用流式细胞术检测PC12细胞凋亡的百分率;测定细胞内罗丹明123(Rhodamine123)的荧光强度,反映细胞线粒体膜电位的改变.结果:莫达非尼能抑制过氧化氢(200 μmol/L)、硝普钠(500μmol/L)和连二亚硫酸钠(2 mmol/L)对PC12细胞的损伤.过氧化氢(200 μmol/L)24h可以诱导PC12细胞凋亡(凋亡率为32.65%).莫达非尼(15,7.5 μmol/L)显著降低凋亡细胞的百分率(凋亡百分率分别为7.95%、15.46%).并且莫达非尼可以抑制过氧化氢引起的线粒体膜电位降低.结论:莫达非尼对PC12细胞有保护作用.能抑制过氧化氢诱导的PC12细胞凋亡,这一作用可能与其抑制过氧化氢引起的线粒体膜电位降低有关.

Inhibition of Hydrogen Peroxide-induced PC12 Cell Apoptosis by Modafinil

AIM: To study the inhibition of hydrogen peroxide-induced PC12 cell apoptosis by modafinil and its mechanism. METHOD: PC12 cell survival was measured by MTT assay. The percentage of apoptosis was monitored with flow cytometry. By measuring the intracellular rhodamine123(Rh123)fluorescence density,we evaluated the change of PC12 cell mitochondrial membrane potential (mmp) induced by hydrogen peroxide. RESULT:Modafinil attenuated the cytotoxic effect of hydrogen peroxide (200 μmol/L),sodium nitoprusside (500 μmol/L) and sodium dithionite (2mmol/L). Hydrogen peroxide (200 μmol/L,24 h) induced apoptosis in PC12 cells.PC12 cells were preconditioned with modafinil, then the percentage of apoptosis in the cells was decreased significantly to 7.95%,15.46%,and 17.52% from 32.65%. Modafinil inhibited the mmp decrease in PC12 cells induced by hydrogen peroxide. CONCLUSION:Modafinil prevented PC12 cell from apoptosis induced by hydrogen peroxide,and the mechanism might be associated with its effect of increasing the mitochondrial membrane potential of PC12 cell.