RP-HPLC法测定正柴胡饮颗粒中橙皮苷的含量

目的应用反相高效液相色谱法测定正柴胡饮颗粒中橙皮苷的含量。方法色谱柱为phenomenex-ODS（250×4．6mm，5μm），柱温30℃，流动相为乙腈-0．2％磷酸溶液（20：80），检测波长283nm，流速1．0mL·min^-1.结果橙皮苷在0．02208μg-4．416μg（r=0．99999）范围内线性关系良好。平均回收率96．85％，RSD为2．29％。结论本法操作简便、结果准确．重现性好，可作为正柴胡饮颗粒中橙皮苷含量的质量控制方法。

Determination of hesperidin's content in Zhengchaihuyin granules by RP-HPLC

OBJECTIVE To develop a reversed-phase HPLC method of determining the content of hesperidin in Zhengchaihuyin granules. METHODS The HPLC system consisted of the phenomenex-ODS （250mm × 4.6mm, 5μm） and acetonitrile-0.2% phosphoric acid solution（20 ： 80） as the mobile phase at a flow rate of 1.0mL· min^-1. The detection wavelength was at 283 nm and the column temperature was 30℃. RESULTS The measurement proved to be linear over the range of 0. 02208μg- 4. 416μg （r = 0. 99999） for hesperidin. The average recovery was 96.85 % with RSD of 2.29 %. CONCLUSION The method is simple, accurate, good repeatability and can be used for the quality detection of hesperidin in Zhengchaihuyin granules.