乙型肝炎病毒前S1抗原检测在临床中的应用 |
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引用本文: | 丁晓琳,张伦军,刘蕴霞,陈庆书,汪蔷华. 乙型肝炎病毒前S1抗原检测在临床中的应用[J]. 中华全科医学, 2012, 0(1): 109-110 |
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作者姓名: | 丁晓琳 张伦军 刘蕴霞 陈庆书 汪蔷华 |
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作者单位: | 安徽省蚌埠医学院第一附属医院检验科 |
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摘 要: | 目的探讨乙型肝炎病毒前S1(Pre-S1)抗原与乙型肝炎病毒DNA(HBV-DNA)、乙型肝炎e抗原(HBeAg)的相关性。方法对168例乙型肝炎患者和50例正常健康人的血清用酶联免疫法(ELISA)进行Pre S1抗原、乙型肝炎病毒血清标志物(HBV-Marker,HBV-M)的检测,用PCR荧光定量法检测HBV-DNA,比较HBV Pre-S1抗原和HBV-DNA检测结果和相关性。结果 168例HBsAg阳性的乙型肝炎患者中,HBeAg阳性率为57.1%,Pre-S1抗原的阳性率为76.7%,HBV-DNA阳性率为70.2%,Pre-S1抗原和HBV-DNA检测结果两者比较差异无统计学意义(P>0.05),且具有很好的相关性,Kappa>0.75;而其在96例HBeAg阳性患者中,Pre-S1抗原的阳性率为93.7%,HBV-DNA阳性率为94.8%。结论 Pre-S1抗原和HBV-DNA有较高的符合率,可作为HBV复制的指标,Pre-S1 Ag较HBeAg更敏感地反应了HBV的存在和复制情况,可起到提示病毒是否复制的补充作用。
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关 键 词: | 乙型肝炎前S1抗原 乙型肝炎病毒DNA 乙型肝炎e抗原 |
Clinical Application of Detecting Pres1ag in Hepatitis B Virus |
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Affiliation: | DING Xiao-lin,ZHANG Lun-jun,LIU Yun-xia,et al.Department of Clinical Labs,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,Anhui,China |
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Abstract: | Objective To investigate the correlation of HBV-DNA,HBeAg with HBV pre-S1 antigen.Methods For the 168 HBV-DNA positive patients and 50 health controls,HBV pre-S1 antigen and HBV-makers were measured by ELISA and HBV-DNA by fluorescence quantitative PCR.The correlation between HBV pre-S1 and HBV-DNA was analyzed.Results In 168 HBsAg positive cases,the positive rate of HBeAg and HBV pre-S1 antigen were 57.1%,76.7% respectively.The difference between positive rate of Pre-S1 and HBV-DNA was not significant(P>0.05).In 96 HBeAg positive cases,the positive rate of HBV pre-S1 antigen was 93.7%,the positive rate of HBV-DNA was 94.8%;there was no statistical difference between the positive rate of the two indexes.Conclusion HBV pre S1 antigen was consistent with HBV-DNA,they can be used as sensitive markers reflecting the replication of hepatitis B virus in clinical work. |
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Keywords: | HBV pre-S1 antigen HBV-DNA HbeAg |
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