乙型肝炎病毒前S1抗原检测在临床中的应用

目的探讨乙型肝炎病毒前S1(Pre-S1)抗原与乙型肝炎病毒DNA(HBV-DNA)、乙型肝炎e抗原(HBeAg)的相关性。方法对168例乙型肝炎患者和50例正常健康人的血清用酶联免疫法(ELISA)进行Pre S1抗原、乙型肝炎病毒血清标志物(HBV-Marker,HBV-M)的检测,用PCR荧光定量法检测HBV-DNA,比较HBV Pre-S1抗原和HBV-DNA检测结果和相关性。结果 168例HBsAg阳性的乙型肝炎患者中,HBeAg阳性率为57.1%,Pre-S1抗原的阳性率为76.7%,HBV-DNA阳性率为70.2%,Pre-S1抗原和HBV-DNA检测结果两者比较差异无统计学意义(P>0.05),且具有很好的相关性,Kappa>0.75;而其在96例HBeAg阳性患者中,Pre-S1抗原的阳性率为93.7%,HBV-DNA阳性率为94.8%。结论 Pre-S1抗原和HBV-DNA有较高的符合率,可作为HBV复制的指标,Pre-S1 Ag较HBeAg更敏感地反应了HBV的存在和复制情况,可起到提示病毒是否复制的补充作用。

Clinical Application of Detecting Pres1ag in Hepatitis B Virus

Objective To investigate the correlation of HBV-DNA,HBeAg with HBV pre-S1 antigen.Methods For the 168 HBV-DNA positive patients and 50 health controls,HBV pre-S1 antigen and HBV-makers were measured by ELISA and HBV-DNA by fluorescence quantitative PCR.The correlation between HBV pre-S1 and HBV-DNA was analyzed.Results In 168 HBsAg positive cases,the positive rate of HBeAg and HBV pre-S1 antigen were 57.1%,76.7% respectively.The difference between positive rate of Pre-S1 and HBV-DNA was not significant(P>0.05).In 96 HBeAg positive cases,the positive rate of HBV pre-S1 antigen was 93.7%,the positive rate of HBV-DNA was 94.8%;there was no statistical difference between the positive rate of the two indexes.Conclusion HBV pre S1 antigen was consistent with HBV-DNA,they can be used as sensitive markers reflecting the replication of hepatitis B virus in clinical work.