牙周炎对慢性阻塞性肺疾病大鼠肺损伤的作用研究

目的:应用SD大鼠建立慢性牙周炎(chronic periodontitis,CP)和慢性阻塞性肺疾病(chronic obstructive pulmonarydiseases,COPD)动物模型,探讨CP在COPD发生发展过程中对肺损伤的作用。方法:将40只SD大鼠雌雄各半随机分成4组:正常对照组,CP组,COPD组,COPD伴CP组,每组10只。各组按照实验设计施加相应建模因素,实验10周末分别检查大鼠的龈沟出血指数(SBI)、肺功能状况,ELISA法检测血清中TNF-α和IL-1含量,然后处死大鼠,取材观察牙槽骨吸收程度(ABL)、牙周组织和肺部组织病理学改变,对数据进行统计分析。结果:COPD伴CP组大鼠的肺功能明显降低,龈沟出血指数和牙周组织改变与CP组相似,实验组血清IL-1和TNF-α水平明显高于对照组(P<0.05)。结论:CP在COPD大鼠肺损伤的发生发展过程中具有促进作用。

Investigation of the pathogenesis of lung injury in rats with chronic obstructive pulmonary diseases effected by chronic periodontitis

Objective: To investigate the potential correlation between chronic periodontitis(CP) and chronic obstructive pulmonary diseases(COPD) in SD rats.Methods: Equal genders of SD rats were randomly divided into 4 groups: control group,CP group,COPD group and COPD with CP group(n=10).The rats in each group were imposed into the predesigned appropriate intervention for the construction of disease models.10 weeks after the set-up of the disease models,pulmary function was examined,serum TNF-α and IL-1 were detected by ELISA,sulcus bleeding index(SBI) was observed.Then the animals were sacrificed.The level of alveolar bone loss(ABL) and the histo-pathological changes of periodontal tissue and lung tissue were evaluated.Data were analysed statistically.Results: In CP group and COPD with CP group SBI and ABL were more than in control group and COPD group(P<0.05).Pulmonary function in group COPD with CP was worse than that in group COPD.The levels of serum TNF-α and IL-1 increased in the test groups compared with those in control group,the greatest change was found in group D(P<0.05).Conclusion: CP may be a risk factor of lung injury in COPD.