精浆左旋肉碱与精子密度、活力和活动率关系的研究

目的:研究精浆中左旋肉碱浓度与精子密度、活力和活动率的相关性,探讨肉碱在男性不育发病中的作用。方法:分别选取精液常规检查结果为正常、少精、弱精和少弱精的不育患者12、16、20、16例,以液相色谱-质谱/质谱联用技术检测精浆中左旋肉碱浓度,以化学发光免疫分析法检测精浆中睾酮浓度,结果以SPSS15.0行双变量相关分析分析精浆中肉碱浓度与精子密度、活力和活动率的相关性,并以精浆睾酮浓度为控制变量行肉碱与精子密度的偏相关分析。结果:共有64例患者纳入研究,精浆中总肉碱浓度、游离肉碱浓度及酰基肉碱浓度分别为(91.33±40.49)mg/L、(40.89±24.13)mg/L、(50.44±21.90)mg/L;双变量相关分析结果为精浆中总肉碱浓度与精子密度、活力和活动率的相关系数分别为0.637(P<0.001),0.161(P=0.235),0.114(P=0.370),去除少精组后游离肉碱与活力和活动率的相关系数分别为0.325(P=0.024)和0.316(P=0.029);偏相关分析结果显示在剔除睾酮影响后的精子密度与肉碱的相关性仍有显著统计学意义(r=0.641,P<0.001)。结论:精浆中肉碱浓度与精子密度和活力呈正相关,其中与密度的相关性更明显。

Seminal Plasma Levocarnitine Significantly Correlated with Semen Quality

OBJECTIVE: To investigate the relationship of seminal plasma levocarnitine with sperm concentration, vitality and motility. METHODS: Enrolled in this study were 64 infertile men, who were divided according to the results of routine sperm tests into a normozoospermia (n = 12), an oligozoospermia (n = 16), an asthenozoospermia (n = 20) and an oligoasthenozoospermia group (n = 16). The level of seminal plasma levocarnitine was detected by LC-MS-MS, the concentration of seminal plasma testosterone measured by chemiluminescence immunoassay, the correlation of seminal plasma levocarnitine with sperm concentration, motility and vitality determined by bivariate correlation analysis with SPSS15.0, and so was the correlation between the carnitine and sperm concentration by partial correlation analysis with seminal plasma testosterone as a control variable to exclude the influence of testosterone. RESULTS: The concentrations of total seminal plasma levocarnitine, free seminal plasma levocarnitine and seminal plasma acetolevocarnitine were (91.33 +/- 40.49) mg/L, (40.89 +/- 24.13) mg/L and (50.44 +/- 21.90) mg/L; the Pearson coefficients of correlation of the levocarnitine level with sperm motility, vitality and concentration were 0.161 (P = 0.235), 0.114 (P = 0.370) and 0.637 (P < 0.001), those of free seminal carnitine with sperm motility and vitality were 0.325 (P = 0.024) and 0.316 (P = 0.029), respectively, with the oligozoospermia group excluded, and that of partial correlation between the concentrations of seminal levocarnitine and sperm was 0.641 (P < 0.001). CONCLUSION: The level of seminal plasma levocarnitine is positively correlated with sperm motility and vitality, and more significantly with sperm concentration.