Renal adaptation to dietary amino acid alteration is expressed in immature renal brush border membranes |
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Authors: | R. W. Chesney N. Gusowski S. Lippincitt I. Zelikovic |
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Affiliation: | (1) Department of Pediatrics, Division of Nephrology, University of California, School of Medicine, 95616 Davis, CA, USA |
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Abstract: | The transport of ions and solutes at the epithelial surface of the renal proximal tubule increases during periods of reduced dietary intake and decreases with dietary excess. We have used the sulfur-containing -amino acid, taurine, as a probe of this renal adaptive response to altered dietary sulfur amino acid intake to better understand the mechanisms of renal amino acid reabsorption. There exists an age-related precession of taurine uptake values by brush border membrane vesicles prepared from nursing rats from youngest to oldest. However, despite the immaturity of this transport mechanism, epithelial membranes become able to display a full renal adaptive response to altered sulfur amino acid intake sometime between the 7th and 14th day of life. This adaptive response is expressed in both up regulation and down regulation by means of a change in the initial rate of Na+-taurine cotransport. No changes in the lipid microenvironment of the membrane, as assessed by measurements of membrane fluidity, are evident. The lack of adaptation observed in 7-day-old pups may be due to immaturity of the Na+ transporting mechanism which energizes the uptake of amino acids.Supported in part by NIH grants AM 31682 and 37232 |
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Keywords: | Brush border membranes Membrane fluidity Developmental renal physiology Taurine Amino acid transport |
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