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两种方法测定乙型肝炎病毒血清标志物的比较研究
引用本文:何英爱,姚叶林,刘见欢,何雪环,朱彩嫦,蒲荣. 两种方法测定乙型肝炎病毒血清标志物的比较研究[J]. 检验医学与临床, 2016, 0(Z2): 123-125. DOI: 10.3969/j.issn.1672-9455.2016.26.053
作者姓名:何英爱  姚叶林  刘见欢  何雪环  朱彩嫦  蒲荣
作者单位:广东省东莞市第三人民医院检验科 523326
摘    要:目的:探讨GIGA (金标快速检测板法)检测乙肝五项血清标志物的结果可靠性。方法357例待测血清标本同时采用ELISA(酶联免疫吸附法)和GIGA(金标快速检测板法)进行检测,对检测结果进行方法学分析。结果357例血清标本中,286例GIGA法与ELISA法结果相符,有71例结果不完全一致。36例ELISA法检测 HBsAg阳性标本中,GIGA法有1例未检出。结论乙肝五项血清标志物的结果GIGA法比ELISA法准确性稍低,但GIGA法操作简便,快速,易于观察结果,且GIGA法中HBsAg检测结果与ELISA法基本一致,适用于急诊手术前以及献血前的快速筛查。

关 键 词:乙型肝炎病毒  血清标志物  酶联免疫吸附法  金标快速检测板

ELISA and GIGA method a comparative study of the HBV serum markers
Abstract:Objective To study the reliability of the results of five hepatitis B virus by GICA .Methods ELISA and GICA were used to detect the serum samples from 357 patients ,Methodology analysis was carried out on the test results .Results Of the 357 serum samples ,286 had the same results ,and 71 cases were not completely consistent .ELISA detected HBsAg positive in 36 cases , 1 cases of GICA was not detected .Conclusion The accuracy of GICA is lower than that of ELISA ,but compared with ELISA ,GI-CA is simple ,rapid and easy to observe .And in the detection of HBsAg ,GICA and ELISA are basically the same ,GICA can be used for emergency and simple screening before blood donation .
Keywords:HBV  Serum markers  ELISA  GIGA
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