乳腺肿瘤及癌前病变中基底膜和肌上皮细胞变化的研究

应用免疫过氧化物酶技术研究70例良、恶性乳腺病变基底膜层粘蛋白(Laminin,LN)的分布;通过对连续切片进行偶氮荧光桃红染色,观察乳腺良、恶性病变中肌上皮细胞的改变及其与LN之间的关系。结果显示:正常乳腺组织基底膜呈连续线状,肌上皮细胞为不连续单层环绕腺泡及导管。乳腺良性病变及原位癌未发生浸润时基底膜完整,肌上皮细胞增生。在浸润乳腺癌中基底膜缺损,缺损程度随分化程度降低而增加。各型乳腺癌中均见到癌性肌上皮细胞。在癌性肌上皮细胞附近以及两例恶性肌上皮瘤中基底膜减少或缺失。本研究表明利用免疫组化技术观察基底膜的分布有助于区别原位癌和早期浸润癌。人类肌上皮细胞参与基底膜的合成,当肌上皮细胞发生恶变时,其产生基底膜的功能下降,甚至消失。

Studies of Basement Membrane and Myoepithelial Cell Changes in Benign and Malignant Breast Tumors

Using immunohistochemistry, the distributicn patterns of basement membrane component Laminin were investigated in 70 cases of benign and malignant breast tissue, and compared with myoepittelial cell changes in the series slides deteted by Azophloxine Stain. In normal breast tissue, in benign breast lesions, and in situ malignancies the basement memrbane always surroud the duct end the tabules, whereas in invasive breast carcinomas it is often absent. In intraductal mammary carcinomas, fragmentation and absence of basement membrane was found in areas of microinvasion. In normal breast tissue, myoepithelial cells appeared in a discontinuous layer between luminal epithelial cells and basement membrane. In benign lesions, it was increased in number. In malignant tumors, a more or less atypical myoepithelial cells were obseved, where the basement membrane was always absent. In two case of malignant myoepithelioma of the breast, basement membrane was rare. The results suggest that immunohistochemical detection of basement membrane can be of value for differential diagnosis between carcinoma in situ and early invasive carcinoma in breast tissue. Myoepithelial cells are involved in basement membrane production. When myoepithelial cells becomes maligant it's finction of broducting basement membrane protains is decreased