中心体α-微管蛋白、γ-微管蛋白在脑胶质瘤中的表达及其与Survivin表达的相关性研究

目的 探讨中心体α-微管蛋白和γ-微管蛋白在脑胶质瘤中的表达，及其与凋亡抑制基因Survivin表达的相关性。方法 应用免疫组织化学方法检测51例脑胶质瘤组织中α-微管蛋白、Survivin蛋白的表达，免疫荧光染色方法检测γ 微管蛋白的表达，并分析其之间的相关性。结果 脑胶质瘤高级别组（Ⅲ～Ⅳ级）α-微管蛋白、γ-微管蛋白及Survivin蛋白表达阳性率（88.0%、80.0%、92.0%）明显高于低级别组（Ⅰ、Ⅱ级）（22.2%、47.1%；11.1%、35.3%；33.3%、52.9%），且差异具有统计学意义(P均＜0.0125)。α-微管蛋白、γ-微管蛋白与Survivin表达之间均呈正相关（r分别为0.440?4、0.547?8，P＜0.05)。结论 α-微管蛋白、γ-微管蛋白和Survivin在胶质瘤中的表达随恶性程度升高而增加，且相互之间均呈正相关。微管蛋白与Survivin的相互作用可能是胶质瘤发生发展过程中的重要事件。

Expressions of α-Tubulin and γ-Tubulin in human Glioma and their relation with Survivin

Objective To evaluate expressions of α-Tubulin and γ-Tubulin in human glioma and to investigate the correlation between Tubulin and Survivin. Methods The immunohistochemical method or immunofluorescence technique was used to determine expressions of α-Tubulin, γ-Tubulin and Survivin in specimens of glioma. The relation of α-Tubulin and γ-Tubulin to Survivin was analyzed using statistic methods. Results  Positive rates of α-Tubulin, γ-Tubulin and Survivin were significantly higher in high grade glioma than in low grade(P＜0.0125). Expressions of α-Tubulin and γ-Tubulin were positively related to expression of Survivin (P＜0.05). Conclusion Malignancy of glioma is related to expressions of α-Tubulin, γ-Tubulin and Survivin. Interactions between Tubulin and Survivin may be important signals of development and prognosis in human glioma.