钴铬钼微孔表面人工髋关节材料细菌源性生物腐蚀的初步研究

作者针对由于绿脓杆菌感染造成焊接型ＰＣＡ全髋置换失败，再次手术中发现关节材料表面有绿色锈斑、金属珠粒易脱落等问题，采用同种材料和工艺制成的金属试样进行细菌学研究。细菌培养分为需氧培养和厌氧培养两大组，菌株为绿脓、金葡、大肠、产气荚膜等六种常见致病菌，接种成单一菌落和混合菌落，金属试样分别浸泡在含有上述菌落的普通肉汤培养基（用于需氧培养）和庖肉培养基（用于厌氧培养）中。半年后取出，在实体工具镜下观察金属材料表面的情况。结果发现，需氧培养中产绿色绿脓色素的绿脓杆菌和厌氧培养下的大肠杆菌可以在金属材料表面形成点蚀及色斑，与临床所见相符。这一结果说明，在感染的全髋置换病例中，人工关节材料的细菌源性生物腐蚀确实存在，并且是手术失败的原因之一。

Microbial Corrosion of Porous Co-Cr Alloy Samples-a Preliminary inVitro Study

In order to confirm the presence of pyocyanic infection in the green rust spots over the surface of welding type porous Co-Cr Alloy (PCA) hip prothesis in the failed THA cases, the authors performed a series of bacteriological immersion tests under the aerobic and anaerobic culture conditions. The selected species of bacteria consisted of Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus coli, Bacillus perfringens etc. The above-mentioned bacteria specimens were added to the single colony and the mixed colony for the tests. The welding type PCA samples were immersed in broth bouillon and chopped meat media with the above colonies. The samples were procured and the surface changes were observed under the microscope six months later. The results showed that the spotty corrosion occurred only on the bead surfaces of the samples that were immersed in the media with Pseudomonas aeruginosa under aerobic culture conaition and Bacilus coli under anaerobic condition. The results confirmed the fact that microbial corrosion of PCA exists and is one of the causes of failure of infected THA.