不同的抗原修复方法对肺癌组织TTF-1、P53、SPB等6种抗体表达的影响

目的探讨不同的修复方法配搭不同修复液对肺癌组织TTF-1、P53、SPB等6种抗体表达的阳性数量及强度是否存在影响。方法10例肺癌患者标本,分别用微波、高压配搭柠檬酸缓冲液(pH6.0)、EDTA缓冲液(pH8.0、pH9.0)处理,分别滴加一抗,采用二步法,进行免疫组化标记,分别由两位病理医师进行独立看片,并进行结果比较。结果不同的修复方法配搭不同修复液对这些抗体表达的阳性数量及强度存在影响。结论微波配搭柠檬酸缓冲液对易表达抗体的染色效果佳;高压配搭EDTA缓冲液(pH8.0)对显示细胞核染色效果佳,高压配搭檬酸缓冲液(pH6.0)对显示难表达抗体的细胞浆、细胞膜染色效果佳。

The influence of antigen retrieval on the results of immunohistochemical staining

Objective To evaluate the influence of combinations of different antigan retrieval methods and solutions basing on the results of the expressions of the antibodies. Methods 10 specimens of lung cancer were processed with microwave, high-pressure matched with citrate buffer solution (pH6.0), EDTA buffer (pHS.0), EDTA buffer (pH9.0) respectively, and then carried out immunohistochemically by Maxvision two-step system. All the sections were observed separately by two independent pathologists, and the results were compared. Results The antibody expression was influenced by different combinations of different retrieval methods and solutions. Conclusion Combinations of microwave retrieval and citric acid buffer, high-pressure retrieval and EDTA buffer(pH8.0) and high-pressure retrieval and citric acid buffer (pH6.0) were good for staining of easily-expressing antibodies in cytoplasm and cellular membrane staining of antibody in cell nucleus and staining of difficult-expressed antibodies in cytoplasm and cell membrane respectively.