Platelet‐Derived Growth Factor‐BB Release Profile in Gingival Crevicular Fluid After Use of Marginal Periosteal Pedicle Graft as an Autogenous Guided Tissue Membrane to Treat Localized Intrabony Defects |
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Authors: | Ahmed Yousef Gamal Ossama Sayed Ahmed El‐Shal Mahmoud Mohammed El‐Aasara Eman Mohammed Fakhry |
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Institution: | 1. Department of Periodontology, Faculty of Dental Medicine, Al Azhar University, Cairo, Egypt.;2. Regional Center for Mycology and Biotechnology, Al Azhar University. |
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Abstract: | Background: The aim of this study is to evaluate levels of platelet‐derived growth factor‐BB (PDGF‐BB) in gingival crevicular fluid (GCF) during the early stages of healing for sites treated by marginal periosteal pedicle (MPP) graft as an autogenous guided tissue membrane compared to that of the control open flap debridement (OFD). Methods: Fifteen non‐smoking patients (13 males and 2 females) with severe chronic periodontitis participated in this prospective, controlled, masked trial. Each subject contributed matched pairs of 2‐ or 3‐walled intrabony interproximal defects in premolar or molar teeth. Interproximal contralateral defects were randomly assigned to either the MPP group 1 or control OFD group 2. GCF samples were collected at 1, 3, 7, 14, and 30 days after surgery. PDGF‐BB in the GCF samples was measured using a human PDGF‐BB enzyme‐linked immunosorbent assay kit. Results: In both MPP and OFD, PDGF‐BB concentrations peaked in the samples obtained during the early postoperative days (days 2 and 3) and decreased sharply in the samples obtained 7, 14, and 30 days post‐surgery. Conclusion: Periosteal coverage of periodontal defects is not associated with a significant increase in PDGF‐BB levels. |
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Keywords: | Gingival crevicular fluid intercellular signaling peptides and proteins marginal periosteal pedicle periodontal guided tissue regeneration platelet derived growth factor tissue engineering |
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