血管内皮生长因子抗体对胶质瘤细胞凋亡的影响

目的 观察血管内皮生长因子(VEGF)抗体对大鼠C6胶质瘤细胞凋亡的影响及对肿瘤生长的抑制作用。方法 皮下接种建立异位大鼠C6胶质瘤模型，实验组每3d局部应用VEGF抗体，对照组局部应用PBS溶液，每5d量取肿瘤的长径和宽径，15d后处死动物，称取肿瘤湿重，肿瘤组织行H—E及TUNEL法原位凋亡检测。结果 实验组动物肿瘤生长速度明显减慢，光镜下观察实验组肿瘤标本见到较多的坏死灶，TUNEL法原位凋亡检测发现实验组肿瘤组织内肿瘤细胞凋亡。结论 应用VEGF抗体后大鼠C6胶质瘤生长速度减慢，肿瘤细胞发生凋亡。抗血管形成疗法不仅能使肿瘤坏死增加，而且可以诱导肿瘤细胞凋亡。

Affect of VEGF antibody on the cell apoptosis of C6 gliomas

Objective To observe the affect of vascular endothelial growth factor (VEGF) antibody on the cell apoptosis and growth of rat C6 glioma. Methods We established the heterotopic subcutaneous C6 glioma model, VEGF antibody was locally injected into the tumor of experiment group. The lenth, width and weight of the tumor were measured every 5 day, and animals were sacrificed and weighted after 15 days, H-E staining and in situ apoptosis (TUNEL) were detected. Results Tumor of the experiment group growed more slow, tumor necrotic area increased in H-E stains, and we found apoptosis of tumor cells by means of In situ apoptosis(TUNEL) detection. Conclusion C6 glioma grows slowly after the application of VEGF antibody and tumor cells apoptosis happens; blocking of VEGF-VEGFR signal conduction way can induce necrosis and apoptosis of tumor cells.