环氧合酶-2、基质金属蛋白酶-9和核增殖抗原在胃癌组织中的表达及临床意义

目的 探讨环氧合酶-2(COX-2)、基质金属蛋白酶-9(MMP-9)及核增殖抗原(Ki67)在胃癌中的表达与胃癌发生、浸润和转移的关系.方法 选择2003年1月至2005年12月手术切除、病理证实的胃癌存档蜡块标本58例,其中男37例,女21例.年龄31～76岁,中位年龄58.2岁.另取上述胃癌根治术患者距肿瘤5～6 cm的癌旁组织作对照.采用免疫组化法检测胃癌组织中COX2、MMP-9、Ki67的表达.结果 COX-2、MMP-9在胃癌组织中的表达率分别为82.76%和68.9%,均高于对照组(37.93%和24.14%,P＜0.01).COX-2、MMP-9的表达与胃癌患者性别、年龄、部位及大小无相关性(P＞0.05),与胃癌浸润深度、淋巴结转移及TNM分期有关(P＜0.05),MMP-9还与胃癌分化程度相关(P＜0.05).COX-2与MMP-9在胃癌组织中的表达有相关性(P＜0.05,C=0.359).MMP-9、COX-2表达阳性者Ki67表达水平高于阴性者,两者间差异有统计学意义(P＜0.01).结论 COX-2、MMP-9、Ki67在胃癌浸润、转移过程中起重要作用,共同促进肿瘤的发生、发展.

Expressions of cyclooxygenase-2,matrix metalloproteinases-9 and Ki67 in tissues of gastric cancer and their clinical significance

Objective To investigate the association of expressions of cyclooxygenase-2(COX-2),matrix metanoproteinases-9(MMp-9)and Ki67 with occurrence,infiltration and metastasis of gastric cancer.Methods Fifty-eight surgically obtained and pathologically performed cancerous tissues were collected between Jan.2003 and Dec.2005.Among them,37 were males and 21 were females with mean age of 58.2 years(ranged from 31 to 76 years).Fifty-eight paracancerous tissues(5-6 cm apart form the cancer)were taken as controls.The expressions of COX-2,MMP-9 and Ki67 in cancerous or paracancerous tissues were examined by immunohistochemistry.Results The expressions of COX-2 and MMP-9 in cancerous tissues(82.76%and 68.9%,respectively)were higher than those in controls(37.93%and 24.14%,respectively,P＜0.01).The expressions of COX-2 and MMP-9 were not associated with sex,age,location and size of gastric cancer(P＞0.05),but were correlated with the depth of lymph node infiltration.metastasis and TNM stage(P＜0.05).The expression of MMP-9 was also associated with the degree of differentiation of gastric cancer(P＜0.05).There was a correlation between COX-2 and MMp-9 expressions in gastric cancer tissues(P＜0.05,C=0.359).The expression of Ki67 in tissues with positive expressions of COX-2 and MMp-9 was higher than that in negative ones(P＜0.O 1).Conclusion The COX-2,MMP-9 and Ki67 play important roles in metastasis and infiltration,which promote occurrence and development of gastric cancer.