Cytomegalovirus- and interferon-related effects on human endothelial cells Cytomegalovirus infection reduces upregulation of HLA class II antigen expression after treatment with interferon-γ |
| |
Authors: | Martin Scholz Annette Hamann Roman A Blaheta Marcus K H Auth Albrecht Encke and Bernd H Markus |
| |
Institution: | From the Department of Surgery, Johann Wolfgang Goethe University, Frankfurt, Germany |
| |
Abstract: | Cultured human umbilical vein endothelial cells (HUVEs) were infected with human cytomegalovirus (HCMV) strain AD169. Up to 50% HUVEs proved to be positive for HCMV early nuclear antigens 24 hours after inoculation with virus. Following infection kinetics of surface expression of HLA class I and II, intercellular adhesion molecule (ICAM-1) and endothelial lymphocyte adhesion molecule (ELAM-1) on HUVEs were investigated by means of flow cytometry. A slight increase in HLA class I expression was observed, whereas expression of HLA class II (DR, DP, DQ) antigens was not induced by infection with HCMV. Furthermore, when compared with uninfected cells treated with interferon-γ (IFN-γ), reduced enhancement of HLA-DR expression was conspicuous in HCMV-infected cells treated with IFN-γ. There is evidence that only a portion of HUVE is affected in its ability to upregulate HLA class II antigens. While expression of ICAM-1 was found to be enhanced between 8 and 20 hours after infection with a maximum at 12 hours after infection, no modulation of ELAM-1 was seen. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|