吴茱萸碱抑制胃癌SGC-7901细胞生长及诱导凋亡作用的研究

目的观察吴茱萸碱对胃癌SGC-7901细胞凋亡的生长抑制作用,并探讨可能的分子机制。方法体外培养人胃癌SGC-7901细胞,分别用0.5、1.0、1.5μmol/L吴茱萸碱及吴茱萸碱＋20μmol/L胱天蛋白酶抑制剂（Z-VAD-FMK）作用于SGC-7901细胞12、24和36 h。四唑盐（MTT）比色法观察吴茱萸碱对SGC-7901细胞增殖活性的影响。采用膜联蛋白V-异硫氰酸荧光素/碘化丙啶（Annexin V-FITC/PI）双染细胞,流式细胞仪检测SGC-7901细胞凋亡的情况。结果吴茱萸碱能抑制SGC-7901细胞增殖,MTT结果显示具有时间和剂量的依赖性;流式细胞仪结果显示吴茱萸碱可诱导SGC-7901细胞凋亡,且随剂量和时间的增加作用增强;Z-VAD-FMK可部分抑制吴茱萸碱诱导该细胞的凋亡作用。结论吴茱萸碱能抑制人胃癌SGC-7901细胞增殖并诱导其凋亡,且在加入Z-VAD-FMK后吴茱萸碱仍可诱导其凋亡,但作用减弱。该研究结果提示吴茱萸碱诱导胃癌SGC-7901细胞凋亡除胱天蛋白酶途径外,还存在其他的诱导凋亡途径。

Study of evodiamine on proliferation inhibition and apoptosis induction in human gastric cancer cell line SGC-7901

Objective To observe the effect of evodiamine on proliferation inhibition and apoptosis induction to SGC-7901 cells in human gastric cancer and investigate the possible molecular mechanism.Methods The SGC-7901 cells were cultured in vitro with 0.5,1.0,1.5 μmol/L evodiamine respectively for 12,24 and 36 h.Both evodiamine and caspase inhibitor Z-VAD-FMK were also added into the cultured SGC-7901 cells for 12,24 and 36 h.The cells were stained by Annexin V-FITC/PI.Monotetrazolium（MTT） and flow cytometry were performed to detect the situation of evodiamine on cellular proliferation and apoptosis.Results MTT detection results indicated that the proliferation of SGC-7901 cells was inhibited by evodiamine with time and dose dependence.Flow cytometry results showed that evodiamine induced the apoptosis of SGC-7901 cells more significently with the increasing of time and dose dependence,and the caspase inhibitor Z-VAD-FMK could not entirely inhibit the apoptosis.Conclusions The proliferation of SGC-7901 cells may be inhibited by evodiamine,and its apoptosis is also induced by evodiamine.Evodiamine with Z-VAD-FMK can also induce SGC-7901 cell apoptosis.However,without Z-VAD-FMK,evodiamine can inhibit SGC-7901 cell proliferation and induce cell apoptosis more obviously.The results indicate that there exists other apoptosis pathway except of caspase pathway,for which evodiamine induces SGC-7901 cells in human gastric cancer.