| 基于HPV16的新型伪病毒对DNA特异性B细胞的杀伤研究 |
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| 引用本文： | 吴佩泽,杨明会. 基于HPV16的新型伪病毒对DNA特异性B细胞的杀伤研究[J]. 中国现代医药杂志, 2008, 10(5): 1-3 |
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| 作者姓名： | 吴佩泽 杨明会 |
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| 作者单位： | 中国人民解放军总医院内科临床部,北京,100853 |
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| 摘 要： | 目的 构建基于人乳头状病毒(human papilloma virus,HPV)16型的新型伪病毒,并检测其对DNA特异性B细胞(R4A)的杀伤效应.方法 利用昆虫杆状病毒表达系统表达病毒蛋白,在体外变性与复性过程中将病毒蛋白包装B细胞特异性启动子IgK控制下的白喉毒素(Diphtheriatoxin,DT)A链(DT-A)基因表达型质粒DNA,形成伪病毒.转染R4A细胞,荧光显微镜和流式细胞仪检测其杀伤效应.酶联免疫法检测R4A细胞分泌抗ds-DNA抗体的滴度.结果 转染R4A细胞48 h后,荧光显微镜和流式细胞仪成功检测到新型伪病毒对R4A细胞的杀伤效应,R4A细胞分泌抗ds-DNA抗体的滴度明显降低.结论 基于HPV16的新型伪病毒能有效特异杀伤R4A细胞,并降低抗ds-DNA抗体的滴度,为系统性红斑狼疮的治疗提供了理想的策略.
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| 关 键 词： | 人乳头状瘤病毒16型 伪病毒 系统性红斑狼疮 |
Study on kill effect of pseudovirus of human papilloma virus type 16 on DNA specific B cells |
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| Wu Peize,Yang Minghui. Study on kill effect of pseudovirus of human papilloma virus type 16 on DNA specific B cells[J]. Modern Medicine Journal of China, 2008, 10(5): 1-3 |
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| Authors: | Wu Peize Yang Minghui |
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| Affiliation: | Wu Peize,Yang Minghui. Department of Clinical Medicine,Chinese PLA General Hospital,Beijing 100853 |
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| Abstract: | Objective To construct pseudovirus of human papilloma virus type 16 (HPV16) and to examine its kill activity on DNA specific B cells( R4A). Methods Virus protein was expressed by Bac-to-Bac expression system,DT-Aexpression plasmid together with virus protein were formed into pseudovirus in the course of degeneration and regeneration in vitro. R4A cells were transfected with pseudovirus,and kill activity was detected by fluoroscope and FCM. The titer of ds-DNA antibody was also detected by ELISA assay. Resul... |
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| Keywords: | Human papillomavirus type 16 Pseudovirus SLE |
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