| 阿霉素诱导细胞凋亡过程中的信号传递 |
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| 引用本文： | 金晓明 张引成. 阿霉素诱导细胞凋亡过程中的信号传递[J]. 肿瘤研究与临床, 1999, 11(5): 316-317 |
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| 作者姓名： | 金晓明 张引成 |
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| 作者单位： | 西安医科大学口腔医院!西安市西五路７１０００４ |
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| 摘 要： | 目的探讨阿霉素诱导人涎腺癌细胞凋亡的分子机制。方法通过光镜和电镜观察不同作用时间后细胞的形态学改变,ＤＮＡ琼脂糖凝胶电泳,流式细胞仪分析ＤＮＡ含量和细胞周期,并检测细胞内Ｃａ(２＋)浓度的变化。结果阿霉素作用于ｍＥｃ－１细胞后出现了细胞凋亡的特征,细胞内Ｃａ(２＋)浓度明显增力。。结论Ｃａ(２＋)参与细胞凋亡过程可能是阿霉素诱导ＩＥＣ－１细胞凋亡的机制之一。
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| 关 键 词： | 细胞凋亡 人涎腺癌细胞系 信号传递 |
THE SIGNAL TRANSDUCTION OF APOPTOSIS INDUCED BY ADRIAMYCIN |
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| Jin Xiao ming,Zhang Yin cheng,Li Ya xin,et al. THE SIGNAL TRANSDUCTION OF APOPTOSIS INDUCED BY ADRIAMYCIN[J]. Cancer Research and Clinic, 1999, 11(5): 316-317 |
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| Authors: | Jin Xiao ming Zhang Yin cheng Li Ya xin et al |
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| Abstract: | Objective To explore whether or not driamycin inhibits mEC cells by apoptosis. MethodsAdriamycin was used for mucoepidermoid carcinoma cells mEC-l. Morphologic changes were observed atdifferent times by light and electronic microscope. DNA fragments were shown on agarose gelelectrophoresis. DNA content and cell cycles were analyzed by flow cytometer. Changes of intracellularcalcium ion was monitored by flow cytometer. Results mEC-1 cells presented with classic morphologicfeatures of apoptosis. Intracellular calcium ion was increased. Conclusion Apoptosis followed by thechanges of intracellular calcium ion in human salivary gland mucoepidermoid carcinoma cells is one of themechanisms of adriamycin inhibited tumor cell growth. |
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| Keywords: | Apoptosis Human salivary gland carcinom cell lines Signal transduction |
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