食品中17种氨基酸的二极管阵列—荧光检测器联用分析法

目的　应用两种检测器联用和在线衍生技术测定食品中 17种氨基酸。方法　使用带全自动衍生功能的自动进样器进行在线衍生 ,梯度分离 ,再利用二极管阵列 -荧光检测器串联分析。结果　在线衍生技术克服了离线手工衍生的偏差 ,双检测器联用技术能有效提高定性的可靠性和定量的准确性。结论　该方法测定重复性好 ,灵敏度高 ,定性定量准确 ,结果令人满意

Determination on 17 amino acids in foods with coupled detector technique

ObjectiveTo determine 17 amino acids in foods with coupled detector technique and online derivation technique.[Methods]Auto-injec tor with online d erivation function was used to perform derivation online and isolation. Then ana lysis was made with coupled detector technique.[Results] The o nline derivation t echnique overcomes the deviation of manual operating. The coupled detector techn ique can effectively increase both reliability and accuracy.[Conclusio n]This method has high sensibility and accuracy.