| 酶免疫组化法对肾综合征出血热早期诊断的方法学研究 |
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| 引用本文： | 李闻文,吴水河,阎祖炜,龚道科,戴敢.酶免疫组化法对肾综合征出血热早期诊断的方法学研究[J].中国现代医学杂志,2003,13(13):65-67. |
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| 作者姓名： | 李闻文 吴水河 阎祖炜 龚道科 戴敢 |
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| 作者单位： | 1. 中南大学湘雅医学院检验系临床微生物学免疫学教研室,长沙,410078
2. 卫生部肝胆肠外科研究中心 |
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| 摘 要： | 目的：用酶免疫组化法对肾综合征出血热进行早期诊断。方法：将肾综合征出血热病毒(HFRSV)接种于Vero—E6细胞，培养后制高效价病毒抗原片。应用酶免疫组化技术进行检测。结果：酶免疫组化技术对肾综合征出血热能早期、快速、特异性地诊断。结论：实验证明该法具有早期诊断、快速简便、特异敏感等特点，其检测结果可长期保存等优点，尤其适用于基层医疗单位推广应用。
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| 关 键 词： | 肾综合征出血热 酶免疫组化技术 诊断 |
| 修稿时间： | 2003年1月17日 |
THE METHODOLOGY STUDIES ON THE HISTOCHEMICAL ASSAY DIAGNOSIS OF HIMRRHAGIC FEVER IWTH RENAL SYNDROME BY IMMUNO-ENZYMATIC |
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| Li Wenwew,Wu Shuihe,Yan Zuwei,et al..THE METHODOLOGY STUDIES ON THE HISTOCHEMICAL ASSAY DIAGNOSIS OF HIMRRHAGIC FEVER IWTH RENAL SYNDROME BY IMMUNO-ENZYMATIC[J].China Journal of Modern Medicine,2003,13(13):65-67. |
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| Authors: | Li Wenwew Wu Shuihe Yan Zuwei |
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| Institution: | Li Wenwew,Wu Shuihe,Yan Zuwei,et al. Department of Clinical Microbiology and Immunology of Laboratory Faculty of Xiangya Medical College of Central Southern University,Changsha 410078 |
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| Abstract: | Objective:The early diagnosis of hemorrhagic fever with renal syndrome by immuno-enzymatic histochemical assay. Methods:Hemorrhagic fever with renal syndrome virus(HFRSV) were inoculated with Vero-E6 cell, after culture, the high titer virus antigen slides were made for using to immuno-enzymatic histochemical assay.Results:It is superior in early rapid, specifivity, sensitivity diagnosis. Conclusions:The immuno-enzymatic histochemical assay is superior in early rapid, specifivity, sensitivity and simplicity, long time to store. This method is suitable for basic hospital. |
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| Keywords: | HFRS Immuno-Enzymatic Histochemical Assay Diagnosis |
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