| 妇科止带片中盐酸小檗碱的含量测定 |
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| 引用本文： | 周星,刘文林.妇科止带片中盐酸小檗碱的含量测定[J].高校保健医学研究与实践,2009,6(1):39-41. |
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| 作者姓名： | 周星 刘文林 |
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| 作者单位： | 周星,ZHOU Xing(海南省药物研究所 研究1室,海口,570216);刘文林,LIU Wen-lin(海南制药厂有限公司,研发部,海南,五指山,572200)
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| 摘 要： | 目的建立妇科止带片中盐酸小檗碱的含量测定方法。方法采用高效液相色谱法测定妇科止带片中盐酸小檗碱的含量，色谱柱为Kromasil C18柱，流动相为乙腈-0．1％磷酸溶液（50：50，每100mL加十二烷基硫酸钠0．1g），检测波长为266nm。结果盐酸小檗碱在0．109-0．436μg范围内线性良好（r=0．9998），平均回收率（n=6）为98．3％，RSD为0．9％。结论本含量测定方法可靠、准确，灵敏度高，可用于本品的含量测定。
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| 关 键 词： | 妇科止带片 盐酸小檗碱 高效液相色谱法 |
Determination of Berberine Hydrochloride of Fuke Zhidai Tablets |
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| ZHOU Xing,LIU Wen-lin.Determination of Berberine Hydrochloride of Fuke Zhidai Tablets[J].Health Medicine Research and Practice in Higher Institutions,2009,6(1):39-41. |
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| Authors: | ZHOU Xing LIU Wen-lin |
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| Institution: | 1. The 1 st Research Room, Pharmaceutical Institute of Hainan Province, Haikou 570216, China 2. Department of Research and Development, Hainan pharmaceutical Company Limited, Wuzhishan Hainai 572200,China) |
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| Abstract: | Objective To establish a HPLC method for the determination of berberine hydrochloride in fuke zhidai tablets. Method The content of berberine hydrochloride in Fuke Zhidai tablets was determined on Kro- masil C18 column by HPLC. The mobile phrase was acetonitrile-0.1% phosphoric acid （50 ： 50,add 0.1 g sodium dodecyl sulfate per 100 mL）. The detection wavelength was set at 266 nm. Results A good linear for berberine hydrochloride was in the range of 0. 109-0. 436 μg （r=0. 999 8）. The average recovery（n=6） was 98.3% ,RSD：0.90%. Conclusion This method is reliable, accurate ,sensitive and can be used for the determi- nation of this preparation. |
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| Keywords: | Fuke zhidai tablets Berberine hydrochloride HPLC |
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