micro RNA-155在肾透明细胞癌中的表达及意义

目的：探讨miR‐155在肾透明细胞癌（clear renal cell carcinoma ，CCRCC ）中的表达及意义。方法采用实时定量PCR技术检测18例CCRCC患者新鲜肿瘤组织及癌旁非肿瘤组织中miR‐155的表达情况。结果与配对的癌旁非肿瘤组织相比，肿瘤组织中miR‐155的表达在16例（88.9％）的样本中上调，在2例样本中表达下调，其中CCRCC中miR‐155的相对表达量为2.08～26.05，平均（12.70±6.52），癌旁非肿瘤组织中相对表达量为1.00～6.79，平均为（2.82±1.25），改变有显著统计学差异（ P＜0.05）。根据病理分级样分组，高分化组 miR‐155的相对含量为（11.43±6.18），中‐低分化组为（14.33±7.04），结果有显著统计学差异（P＜0.05）。结论 miR‐155表达上调可能与CCRCC的发病机制相关，其表达水平与Fuhr‐man病理分级密切相关。

The expression and value of miR-155 in clear renal cell carcinoma

Objective To explore the functional role and mechanism of miR‐155 in the development of clear cell renal cell car‐cinoma (CCRCC) .Methods MiR‐155 expression was quantified in renal cancers and matched adjacent non tumor tissues using quantitative real‐time PCR (RT‐PCR) .Results MiR‐155 expressions were up‐regulated in 16 renal cancer tissues (88.9% ) and down‐regulated in 2 renal cancer tissues .Expression level of miR‐155 in renal cancer tissues was significantly higher than that in adjacent normal tissues (12.70 ± 6.52 vs 2.82 ± 1.25 ,P<0.05) and higher expression of miR‐155 was observed in cases with higher stages (11.43 ± 6.18 vs 14.33 ± 7.04 ,P<0.05) .Conclusion Upregulation of miR‐155 may be associated with the path‐ogenesis of CCRCC ,and the expression level is closely related with the Fuhrman pathological grading .