c-kit基因及其靶体SCF在卵巢上皮性肿瘤中的表达

目的:研究c-kit基因及其靶体干细胞因子(stem cell factor,SCF)在卵巢上皮性肿瘤的表达及临床意义,探讨其在卵巢上皮性癌发生发展中的可能作用。方法:免疫组化SP法测定10例正常卵巢组织,20例卵巢良性上皮性肿瘤,16例卵巢交界性上皮性肿瘤,58例卵巢上皮性癌标本中c-kit、SCF蛋白的表达。结果:(1)卵巢上皮性癌中c-kit蛋白阳性表达率为63.79%,明显高于正常卵巢组织(0%)及卵巢良性上皮性肿瘤(10.00%),差异有显著性(P<0.01);卵巢交界性上皮性肿瘤中c-kit阳性表达率为43.75%(7/16),也高于正常卵巢组织及卵巢良性上皮性肿瘤,差异有显著性(P<0.05);(2)卵巢上皮性癌中,低分化组的c-kit蛋白阳性表达率高于高、中分化组(P<0.05),c-kit蛋白的阳性表达率随FIGO分期的进展及淋巴结转移而升高(P<0.05);(3)c-kit阳性患者的预后比c-kit阴性患者差(P<0.05);(4)SCF在正常卵巢上皮、卵巢良性上皮性肿瘤、卵巢交界性上皮性肿瘤、卵巢上皮性癌中的阳性表达率分别为30.00%、35.00%、62.50%和74.14%,卵巢上皮性癌的阳性表达率显著高于正常卵巢组织及卵巢良性上皮性肿瘤(均P<0.01)。卵巢癌低分化组SCF的阳性表达率显著高于高、中分化组(P<0.05)。且随FIGO分期的进展及淋巴结转移而升高(P<0.05);(5)c-kit与SCF表达具有明显相关性(r=0.302,P<0.05)。结论:(1)c-kit、SCF表达异常可能在卵巢上皮性癌的发生发展中起重要作用;(2)c-kit、SCF在卵巢上皮性癌中的表达具有相关性,SCF作为c-kit的配体,可促使c-kit活化,两者具有协同作用(3)c-kit蛋白表达与卵巢上皮性癌患者的预后有关,可作为判断卵巢上皮性癌患者预后的指标之一。

The expression and clinical significance of c-kit and SCF in ovarian epithelial neoplasms

Objective:To investigate the expressions of c-kit and SCF in ovarian epithelial neoplasms and their clinical significance in the development of ovarian epithelial carcinoma.Methods:Expressions of c-kit and SCF proteins were retrospectively studied with immunohistochemistry in paraffin sections from 10 normal ovarian tissues,20 benign ovarian epithelial tumors,16 ovarian borderline epithelial tumors,and 58 ovarian epithelial carcinomas.Results:(1)The positive expression rate of c-kit in ovarian epithelial carcinomas was 63.79%,it was statistically higher than normal ovarian(0%) and benign ovarian epithelial tumors(10.00%)(P<0.01).The positive expression rate of c-kit in ovarian borderline epithelial tumors was 43.75%,it was also statistically higher than those in normal ovarian and benign ovarian epithelial tumors(P<0.05).(2)In ovarian epithelial carcinoma,the positive expression of c-kit in poor differentiated group was higher than that in well and mediate differentiated group(P<0.05),the positive rate increased in advanced stage and lymphatic metastasis.(3)patients with positive expression of c-kit had a poor prognosis.(4)The positive rate of SCF in normal ovarian tissues,benign ovarian epithelial tumors,ovarian borderline epithelial tumors and ovarian epithelial carcinomas were 30.00%,35.00%,62.50% and 74.14% respectively.The positive expression rate in ovarian epithelial carcinoma was apparently higher than in normal ovarian tissues and benign ovarian epithelial tumors(all P<0.01).Negative correlation was found between the expression level of SCF and histology grades,while positive correlation was observed with FIGO clinical stages and lymph node metastasis.(5)There was significant correlation between the expression of c-kit and SCF.Conclusion:(1)The abnormal expression of c-kit and SCF probably play an important role in the development of ovarian epithelial carcinoma.(2)There is correlation between the expression of c-kit and SCF.SCF may stimulate the activation of c-kit as the ligand of c-kit,and both of them may have complementary effect.(3)The expression of c-kit relates to the poor prognosis of ovarian epithelial carcinoma,and may serve as a prognostic factor.