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| RT-PCR技术在肠道病毒脑膜炎暴发中的应用 | |
| 引用本文： | 马宏,胡宝文,李晓光,赵国华,张锦,王建阳,朱谦,许汴利,谢志强,赵升. RT-PCR技术在肠道病毒脑膜炎暴发中的应用[J]. 医药论坛杂志, 2006, 27(11): 62-64 |
| 作者姓名： | 马宏 胡宝文 李晓光 赵国华 张锦 王建阳 朱谦 许汴利 谢志强 赵升 |
| 作者单位： | 1. 河南省疾病预防控制中心传染病预防控制所,郑州市,450016 2. 信阳市浉河区卫生防疫站,信阳市,464000 3. 信阳市疾病预防控制中心,信阳市,464000 |
| 摘 要： | 目的应用套式RT-PCR确定疫情暴发的病原,为制定预防控制措施提供实验室支持。方法提取患者脑脊液和血清中的病毒RNA,用肠道病毒特异性引物进行套式RT-PCR扩增,检测特异性基因片段。结果14份血清和6份脑脊液标本中分别有2份标本肠道病毒特异性基因片段扩增呈阳性。结论套式RT-PCR检测患者脑脊液或血液中的肠道病毒特异性基因片段具有快速、特异的特点,是一种适合突发疫情处置的快速诊断技术。 |
| 关 键 词： | 病毒性脑炎 暴发流行 肠道病毒 |
| 文章编号： | 1672-3422(2006)11-0062-03 |
| 收稿时间： | 2006-03-06 |
| 修稿时间： | 2006-03-06 |
Application of RT-PCR Technique on Outbreak of Enterovirus Encephalitis |
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| MA Hong, HU Baowen, LI Xiaoguang, et al. Application of RT-PCR Technique on Outbreak of Enterovirus Encephalitis[J]. Journal of Medical Forum, 2006, 27(11): 62-64 | |
| Authors: | MA Hong HU Baowen LI Xiaoguang et al |
| Affiliation: | Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China |
| Abstract: | Objective To determine the pathogen causing outbreak with RT-PCR in order to implement preventive measures.Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT-PCR so as to detect specific gene fragment.Results Respectively 2 samples were positive on specific gene fragment of 14 samples of sera and 6 samples of cerebrospinal fluid.Conclusion RT-PCR is a kind of technique to determine pathogen causing outbreaks since it can be used to detect specific gene fragment in cerebrospinal fluid or blood of patients rapidly. |
| Keywords: | RT-PCR |
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