| HPLC测定S-西酞普兰 |
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| 引用本文： | 杨雪梅,刘旭,严轶琛,徐江平.HPLC测定S-西酞普兰[J].第一军医大学学报,2004,24(6):716-717. |
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| 作者姓名： | 杨雪梅 刘旭 严轶琛 徐江平 |
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| 作者单位： | [1]第一军医大学化学教研室，广东广州510515 [2]第一军医大学药理教研室，广东广州510515 |
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| 摘 要： | 目的 拆分西酞普兰(R，S-citalopram)对映异构体，建立S-西酞普兰的质量检测方法。方法 采用CHIROBIOTIC V手性柱，以甲醇-冰醋酸-三乙胺(100：0．1：0．1)为流动相，检测波长240nm，柱温20℃，流速1．0min/ml。结果 S、R型异构体获得完全分离，S-西酞普兰在10～150μg/ml范围内具有良好线性，(r=0．9991，n=5)。结论 该方法简便、准确，可作为S-西酞普兰含量测定及其R型异构体的含量监控方法。
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| 关 键 词： | 手性拆分 S一西酞谱兰 高效液相色谱法 抗抑郁症药物 |
Content determination of S-citalopram by chiral high-performance liquid chromatography] |
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| Xue-mei Yang,Xu Liu,Yi-chen Yan,Jiang-ping Xu.Content determination of S-citalopram by chiral high-performance liquid chromatography][J].Journal of First Military Medical University,2004,24(6):716-717. |
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| Authors: | Xue-mei Yang Xu Liu Yi-chen Yan Jiang-ping Xu |
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| Institution: | Department of Chemistry, First Military Medical University, Guangzhou 510515, China. |
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| Abstract: | OBJECTIVE: To separate the enantiomers of citalopram and determine the content of S-citalopram using chiral high-performance liquid chromatography (HPLC). METHODS: The chiral column CHIROBIOTIC V was used with the mobile phase using methanol-acetic acid-triethylamine (100:0.1:0.1) at the detection wavelength of 240 nm, column temperature of 20 degrees Celsius and flow rate of 1.0 ml/min. RESULTS: Complete separation of the enantiomers of citalopram was achieved, and S-citalopram exhibited good linearity within the concentration range of 10 to 150 microg/ml (r=0.9991, n=5). CONCLUSION: This method allows accurate quantification of S- and R-citalopram and is well suited for drug interaction investigations. |
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