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| 河南省结核分枝杆菌IS6110DNA指纹图谱特征分析 | |
| 引用本文： | 闫国蕊,程豪为,李为民,腊胜明.河南省结核分枝杆菌IS6110DNA指纹图谱特征分析[J].中国卫生检验杂志,2005,15(9):1033-1034. |
| 作者姓名： | 闫国蕊 程豪为 李为民 腊胜明 |
| 作者单位： | 1. 河南省疾病预防控制中心,郑州,450008 2. 河南省医学科学研究所,郑州,450052 3. 北京结核病胸部肿瘤研究所,北京,101149 |
| 基金项目： | 河南省科技攻关资助项目（0124170504） |
| 摘 要： | 目的：从分子流行病学角度探讨河南省结核分支杆菌的分布特征。方法：样本的获得采用全省范围等比例分层整群随机抽样;构建以IS6110 RFLPDNA指纹方法和以DR为基础的Spoligotyping DNA指纹方法;采用Gel compare4．1软件对DNA指纹图谱进行聚类（cluster）分析。结果：共获得66株可进行IS6110 RFLP DNA指纹分析的结核分支杆菌;其中59．09％（39／66）用Spoligotyping DNA指纹方法鉴定为“北京基因型”菌株。结论：北京基因型结核分支杆菌在河南省呈较高水平的流行。 |
| 关 键 词： | 结核分支杆菌 DNA指纹 Spoligotyping 聚类分析 |
| 文章编号： | 1004-8685（2005）09-1033-02 |
| 收稿时间： | 2005-04-11 |
| 修稿时间： | 2005年4月11日 |
Analysis of DNA fingerprint's features of Mycobacterium Tuberculosis IS6110 in Henan province |
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| Yan Guo-rui,CHENG Hao-wei,Li Wei-min,La Sheng-ming.Analysis of DNA fingerprint's features of Mycobacterium Tuberculosis IS6110 in Henan province[J].Chinses Journal of Health Laboratory Technology,2005,15(9):1033-1034. | |
| Authors: | Yan Guo-rui CHENG Hao-wei Li Wei-min La Sheng-ming |
| Abstract: | Objective:To explore the epidemical distribution characteristics of Mycobacterium tuberculosis isolated from Henan province.Methods:The M.tuberculosis strains were gained by multi-stratified grouping random sampling method from the tuberculosis epidemiology survey in Henan and analyzed by IS6110-based RFLP and DR-based Spoligotyping DNA fingerprinting.These fingerprinting patterns were clustering by Gel compare 4.1 software.Results:Thirty-nine of 66 M.tuberculosis strains from this survey were determined to be "Beijing Genotype" M.tuberculosis strain by DNA fingerprinting analysis.Conclusion:M.tuberculosis strains of Beijing Genotype are prevailing in Henan at present. |
| Keywords: | Mycobacterium Tuberculosis DNA fingerprinting Spoligotyping Cluster analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |