A rapid and efficient method for DNA extraction from paraffin wax embedded tissue for PCR amplification |
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| Authors: | Kevin Morgan Letty Lam Noor Kalsheker |
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| Affiliation: | Division of Clinical Chemistry, Department of Clinical Laboratory Sciences, Queen''s Medical Centre, Nottingham NG7 2UH |
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| Abstract: | DNA from archival, formaldehyde fixed, paraffin wax embedded human tissue, suitable for amplification by the polymerase chain reaction (PCR), was obtained using a microwave method based on the capture of DNA by magnetic beads. Fragments of the α-1-antitrypsin gene (AAT) and the apolipoprotein E gene (APOE) were amplified successfully from human liver and brain tissue, respectively. This procedure provides a more rapid, simple and efficient method for reproducibly obtaining DNA from preserved tissue that has been kept in storage for up to 30 years. |
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