双抗原夹心ELISA检测鼠疫F1抗体技术的应用

目的研究双抗原夹心酶联免疫吸附试验（DAgS—EusA）检测鼠疫F1抗体技术在鼠疫监测中的实用性。方法用DAgS—ELISA和间接血球凝集试验（IHA）微量法对比检测558份标本鼠疫F1抗体。结果IHA检测出阳性33份，DAgS—ELISA检出阳性31份，阳性符合率为90．91％，阴性符合率99．81％，总符合率99．28％，二者检出阳性率分别为5．91％和5．56％，差异无统计学意义（X^2=0．25，P=0．625）。2种方法测定27份鼠疫免疫血清均阳性，IHA微量法的敏感性高于DAgS—ELISA（t=3．023，P=0．006）。结论DAgS—ELISA检测鼠疫F1抗体敏感性低于IHA微量法，但特异性好，无前滞反应，可避免初筛漏检问题。

Application of double antigens sandwich enzyme linked immunosorbent assay (DAgS-ELISA) on the detection of Yersinia pestis F1 antibody

Objective To study the practicability of double antigens sandwich enzyme linked immunosorbent assay （DAgS-ELISA）on the detection of Yersinia pestis F1 antibodies. Methods A total of 558 samples antibodies of anti-F1 antigen were detected by DAgS-ELISA and trace indirect hemagglutination assay （trace-IHA）. Results Thirty three samples were positive tested by IHA, 31 positive by DAgS-ELISA, the positive accordance rate was 90.91%, 99.81% for negative accordance rate, 99.28% for the total accordance rate. The positive rate detected by IHA and DAgS-ELISA were 5.91% and 5.56% respectively, and no statistics difference was found （X2=0.25, P=0.625）. About 27 the immuno-serum were positive detected by IHA and DAgS-ELISA methods, and the sensitivity of IHA test were all higher than that of DAgS-ELISA （t=3.023, P=0.006）. Conclusion Sensitivity of DAgS-ELISA is lower than that of trace-IliA, but its specificity is better and no primary inhibitory phenomena, and could exempt from leak detection in the preliminary screening.