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| 分子生物学技术检测鼠形动物巴尔通体 | |
| 引用本文： | 傅桂明,孙继民,杨章女,杨天赐,斯国静,庞卫龙,龚震宇,刘起勇.分子生物学技术检测鼠形动物巴尔通体[J].中国媒介生物学及控制杂志,2009,20(5):454-456. |
| 作者姓名： | 傅桂明 孙继民 杨章女 杨天赐 斯国静 庞卫龙 龚震宇 刘起勇 |
| 作者单位： | 1. 浙江省疾病预防控制中心病媒生物防制所,杭州,310051 2. 杭州市疾病预防控制中心 3. 天台县疾病预防控制中心 4. 中国疾病预防控制中心传染病预防控制所 |
| 基金项目： | 浙江省医药卫生科学研究基金 |
| 摘 要： | 目的查明鼠形动物巴尔通体感染情况。方法在天台县采用夹夜法捕捉鼠形动物，采集该动物的肝脏，用PCR方法检测DNA，并对阳性产物克隆测序，计算感染情况。结果采集的55份鼠类肝脏阳性25份，其中黑线姬鼠阳性率为48．84％，黄毛鼠为33．33％，所检测到的巴尔通体与B．doshiae最接近。结论天台县鼠形动物有很高的巴尔通体DNA阳性率，存在传播给人的风险，必须采取措施加以控制。 |
| 关 键 词： | 巴尔通体 聚合酶链反应 猫抓病 |
Investigation of Bartonella infection in rats by molecular technology |
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| Institution: | FU Gui-ming, SUN Ji-min, YANG Zhang-nv, YANG Tian-ci, SI Guo-jing, PANG Wei-long, GONG Zhen-yu, LIU Qi-yong(Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China) |
| Abstract: | Objective To investigate the infection of BartoneUa in rat-shape animals by molecular technology. Methods Rat-shape animals were captured by night trap in Tiantai county of Zhejiang province. DNA from rat livers were detected by PCR, and the positive products were sequenced. The infection rate of BartoneUa in rat was calculated. Results There were 25 positive samples among 55 samples collected. The positive rate of Bartonella infection in Apodemus agrarius and Rattus losea were 48.84% and 33.33%, respectively. The sequence was more similar to B.doshiae. Conclusion The infection rate of BartoneUa in rat-shape animals was higher in Tiantai county, and there was risk of transmitting this pathogen to human. The control measure should be figured out. It should take measures to control it. |
| Keywords: | Bartonella Polymerase chain reaction Cat-scratch disease |
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