不同龄期和性别德国小蠊羧酸酯酶、谷胱甘肽S-转移酶、乙酰胆碱酯酶活性研究

目的研究德国小蠊羧酸酯酶、谷胱甘肽S-转移酶、乙酰胆碱酯酶在1、3、5龄若虫、雌雄成虫间的活性变化规律。方法采用分光光度计离体测定酶的活性，应用DPS软件进行统计分析。结果对于羧酸酯酶和乙酰胆碱酯酶，1龄若虫的活性最高，其活性分别为（0．3741±0．0077）μmol／（mg pro·min）和（61．7586±9．2400）nmol/（mg pro·min）。对于若虫，随着龄期的增加，其活性逐渐降低，差异有统计学意义；对于成虫，雄成虫羧酸酯酶活性大于雌成虫，差异也有统计学意义，但对于乙酰胆碱酯酶，差异无统计学意义。而谷胱甘肽S-转移酶的活性在不同龄期及雌雄成虫之间差异不明显。结论德国小蠊1、3、5龄若虫、雌雄成虫体内羧酸酯酶、乙酰胆碱酯酶活性在不同龄期之间差异显著，谷胱甘肽S-转移酶在各个龄期及雌雄成虫之间几乎没有差异。

Research on activities of carboxylesterase,glutathione S-transferase and acetylcholinesterase in different development stages and gender adults of Blattella germanica

Objective To-study activity variance of carboxylesterase, glutathione S-transferase and acetylcholinesterase in different development stages and gender adults of German cockroach （Blattella germanica）. Methods Enzyme activities were measured by speetrophotometry in vitro and the data were analyzed with software DPS. Results The activities of carboxylesterase and acetylcholinesterase in the first-instar nymphae were the highest and the activities values were （0.3741±0.0077 ）Ixmol/（ mg pro. min ） and （61.7586±9.2400）nmol/（mg pro-min）, respectively. The differences among different development instars were significant. The activity of carboxylesterase in male adults was higher than that in female adults, and the difference between them was significant. For acetylcholinesterase, the activity in male adults was higher than that in female adults, but the difference was not significant. The activity of glutathione S-transferase among different development stages and gender adults had almost no difference. Conclusion The activity differences of carboxylesterase and acetyleholinesterase among different development stages and male, female adults were significant whereas that of glutathione S-transferase was not significant.