膝关节骨性关节炎患者血清和滑膜液CC趋化因子配体18浓度及与疾病严重程度的关系

目的:探究膝关节骨关节炎患者血清和滑膜液中CC趋化因子配体18(CC-chemokine ligand 18,CCL18)浓度及与疾病严重程度的关系。方法:纳入100例膝关节炎患者和70例健康对照者。采用Kellgren-Lawrence(KL)分级评估膝关节炎的严重程度;采用ELISA方法检测研究对象血清和滑膜液中CCL18浓度;用Spearman相关分析分析血清与滑膜液CCL18浓度间的相关性,血清、滑膜液CCL18浓度与KL分级间的相关性。结果:膝关节炎患者血清CCL18浓度显著高于健康对照者(54.13±8.9) ng/mL vs (38.97±6.9) ng/mL,P<0.001],KL-4级患者血清CCL18浓度显著高于KL-3级患者(72.26±12.90) ng/mL vs (49.56±10.3) ng/mL,P<0.05],KL-3级患者血清CCL18浓度显著高于KL-2级患者(49.56±10.3) ng/mL vs (45.85±8.9) ng/mL,P<0.05]。KL-4级患者滑膜液CCL18浓度显著高于KL-3级患者(45.43±11.09) ng/mL vs (28.49±7.98) ng/mL,P<0.05];KL-3级患者滑膜液CCL18浓度显著高于KL-2级患者(28.49±7.98) ng/mL vs (27.00±5.43) ng/mL,P<0.05]。血清CCL18与滑膜液CCL18浓度显著正相关(r=0.425,P<0.001),血清和滑膜液CCL18与KL分级显著正相关(r=0.560,P<0.001;r=0.525,P<0.001)。结论:血清和滑膜液中CCL18浓度与膝关节炎严重程度相关。

Relationship between the concentration of CC-chemokine ligand 18 in serum and synovial fluid and the severity of knee joint in patients with osteoarthritis

Objective:To determine the relationship between the concentration of CC-chemokine ligand 18(CCL18)in serum and synovial fluid and the severity of knee joint in patients with osteoarthritis.Methods:This study included 100 patients with knee joint osteoarthritis and 70 healthy controls.Disease severity was assessed using the Kellgren-Lawrence grading system and ELISA was used to assess CCL18 concentration in serum and synovial fluid of the study subjects.Spearman correlation analysis was used to analyze the correlation between serum and synovial fluid CCL18 concentration and correlation between serum and synovial fluid CCL18 and KL classification.Results:The serum concentration of CCL18 in patients with knee osteoarthritis was significantly higher than those in healthy controls(54.13±8.9)ng/mL vs(38.97±6.9)ng/mL,P<0.001].The serum concentration of CCL18 in patients with KL-4 was significantly higher than those in KL-3(72.26±12.90)ng/mL vs(49.56±10.3)ng/mL,P<0.05].Patients with grade KL-3 were significantly higher than those with KL-2 grade(49.56±10.3)ng/mL vs(45.85±8.9)ng/mL,P<0.05].Patients with KL-4 grade synovium Liquid CCL18 concentrations were significantly higher than patients with KL-3(45.43±11.09)ng/mL vs(28.49±7.98)ng/mL,P<0.05].Patients with KL-3 were significantly more than those with KL-2(28.49±7.98)ng/mL vs(27.00±5.43)ng/mL,P<0.05].Serum CCL18 levels were positively correlated with synovial fluid CCL-18 levels(r=0.425,P<0.001).Serum and synovial fluid CCL18 levels were positively correlated with KL grades(r=0.560,P<0.001,and r=0.525,P<0.001).Conclusion:The concentration of CCL18 in serum and synovial fluid is related to the severity of knee osteoarthritis.