运动疗法促进坐骨神经损伤小鼠的运动功能恢复

目的:评估运动疗法对坐骨神经损伤小鼠运动功能恢复的促进作用。方法:将60只雄性C57BL/6小鼠随机分为假手术组、坐骨神经损伤组和运动治疗组,每组20只。通过挤压坐骨神经建立坐骨神经损伤小鼠模型。对各组小鼠脚趾形态和坐骨神经功能指数(Sciatic Functional Index,SFI)进行评估。通过免疫荧光染色观察各组小鼠神经纤维的形态及数量。使用透射电子显微镜检查小鼠坐骨神经损伤部位远端的有髓神经纤维数量。使用real-time PCR检测与神经损伤修复相关的基因。结果:与假手术组比较,坐骨神经损伤组小鼠脚趾不能张开,SFI高且下降速度慢,差异有统计学意义(P<0.001)。与坐骨神经损伤组比较,运动治疗组小鼠脚趾张开功能轻度恢复,SFI下降速度快,差异有统计学意义(P<0.001)。免疫荧光染色结果显示:坐骨神经损伤组受损部位远端神经纤维数量少,密度低;而运动治疗组受损部位远端神经纤维数量和密度均升高。电镜结果显示:与假手术组比较,坐骨神经损伤组小鼠受损部位远端有髓神经纤维比例降低,差异有统计学意义(P<0.001);与坐骨神经损伤组比较,运动治疗组小鼠受损部位远端有髓神经纤维比例升高,差异有统计学意义(P<0.01)。Real-time PCR结果显示:与假手术组比较,坐骨神经损伤组BDNF,Mpz和Cdh1基因表达均降低(P<0.01或P<0.001),Artn基因表达升高(P<0.001);与坐骨神经损伤组比较,运动治疗组BDNF,Mpz,Cdh1,Gap-43,cJun基因表达水平升高(P<0.01或P<0.001),Artn基因表达降低(P<0.01)。结论:运动疗法对坐骨神经损伤小鼠的运动功能恢复有促进作用。

Effects of exercise therapy on motor function recovery in mice with sciatic nerve injury

Objective:To evaluate the effect of exercise therapy on motor function recovery in mice with sciatic nerve injury.Methods:A total of 60 male C57 BL/6 mice were randomly divided into a sham operation group,a sciatic nerve injury group and an exercise therapy group,20 rats in each group.A mouse model of sciatic nerve injury was established by squeezing the sciatic nerve.The toe morphology and Sciatic Functional Index(SFI) of mice in each group were evaluated.The morphology and quantity of nerve fibers in each group were observed by immunofluorescence staining.Transmission electron microscopy was used to examine the number of myelinated nerve fibers at the distal end of the mouse sciatic nerve injury site.The genes associated with nerve damage repair were detected by real-time PCR.Results:Compared to the sham group,the mice in the sciatic nerve injury group were not able to open toes,the SFI was higher(P<0.001) and the decline rate was slower.Compared with the sciatic nerve injury group,the toe opening function of mice in the exercise treatment group recovered slightly and SFI decreased rapidly(P<0.001).The results of immunofluorescence staining showed that the number and density of distal nerve fibers of the injured area in the sciatic nerve injury group were low,however,the number and density of the injured distal nerve fibers of the injured area in the exercise treatment group were increased.The results of electron microscopy showed that,compared with the sham group,the ratio of distal myelinated nerve fibers of the injured area in the sciatic nerve injury group was lower(P<0.001);compared with the sciatic nerve injury group,the ratio of distal medullated nerve fibers of the injured area in the exercise treatment group increased(P<0.01).Real-time PCR showed that compared with the sham operation group,BDNF,Mpz and Cdh1 gene expression levels were decreased(P<0.01 or P<0.001) and Artn gene expression level was increased(P<0.001) in the sciatic nerve injury group;compared with the sciatic nerve injury group,BDNF,Mpz,Cdh1,Gap-43 and c Jun gene expression levels were increased(P<0.01 or P<0.001) and Artn gene expression was decreased(P<0.01) in the exercise treatment group.Conclusion:Exercise therapy can promote the recovery of motor function in mice with sciatic nerve injury.