直肠腺癌组织中骨桥蛋白与基质金属蛋白酶-3的表达及其临床意义

目的:探讨骨桥蛋白(osteopontin,OPN)与基质金属蛋白酶-3(matrix metalloproteinase-3,MMP-3)在直肠腺癌组织中的表达及临床意义。方法:采用免疫组织化学EnVision法检测OPN与MMP-3在64例直肠腺癌及癌旁组织中的表达及其相关性,对比分析临床病理特征及随访。结果:OPN与MMP-3在直肠腺癌组织的表达均明显高于癌旁组织,且OPN,MMP-3表达与临床分期、分化程度、淋巴结转移相关(P<0.05);与年龄、性别、浸润深度不相关(P>0.05)。Spearman等级相关分析显示:直肠腺癌中OPN与MMP-3的表达呈正相关(r=0.461,P<0.05)。直肠腺癌临床分期、分化程度及淋巴结转移与OPN,MMP-3阳性表达相关(P<0.05)。OPN与MMP-3阳性患者术后3年总体生存率低于阴性患者(P<0.05)。结论:直肠腺癌OPN与MMP-3表达明显增高,且表达呈正相关,与临床分期、分化程度、淋巴结转移及预后密切相关,有可能成为直肠腺癌重要的生物学标志物,对直肠腺癌诊疗及预后具有重要意义。

Expression of osteopontin and matrix metalloproteinase-3 in rectal adenocarcinoma and its clinical significance

Objective:To investigate the expression and clinical significance of osteopontin(OPN)and matrix metalloproteinase-3(MMP-3)in rectal adenocarcinoma.Methods:Immunohistochemical EnVision method was used to detect the expression and correlation of OPN and MMP-3 in 64 cases of rectal adenocarcinoma and adjacent tissues.The Clinical pathological features were compared and follow-up.Results:The expressions of OPN and MMP-3 in rectal adenocarcinoma were significantly higher than those in adjacent tissues,and the expressions of OPN and MMP-3 were correlated with clinical stage,differentiation and lymph node metastasis(P<0.05).There was no correlation with age,gender,and depth of invasion(P>0.05),and there was no significant difference.Spearman rank correlation analysis showed that OPN was positively correlated with MMP-3 expression in rectal adenocarcinoma(r=0.461,P<0.05).The clinical stage,differentiation degree and lymph node metastasis of rectal adenocarcinoma were correlated with the positive expression of OPN and MMP-3(P<0.05),and there were significant differences.The overall survival rate of OPN and MMP-3 positive patients was lower than that of negative patients(P<0.05).There was a significant difference.Conclusion:The expressions of OPN and MMP-3 in rectal adenocarcinoma are significantly increased,and the expression is positively correlated.It is closely related to clinical stage,differentiation,lymph node metastasis and prognosis,and may be an important biomarker for rectal adenocarcinoma.OPN and MMP-3 are important for the diagnosis and treatment of rectal adenocarcinoma.