HBcAg ELISA法测定的临床分析

目的研究ELISA法测定HB cAg在HBV感染者中的阳性检出率、分布规律及与其他乙肝标志物的关系,探讨其在反映HBV复制及传染性和观察疗效方面的临床价值。方法采用ELISA法对416例HBV感染者进行血清乙肝6项指标测定,按其不同阳性结果分9种模式对比分析。结果416例HBV感染者中HB cAg ELISA法测定阳性总检出率达56.7%,而70例未感染者及65例抗-HB s单项阳性者中无阳性检出。结论ELISA测定血清HB cAg在HBV感染者中的阳性检出具有特异性,并可作为疑有抗-HBe阳性“逆转”为HBeAg阳性者的筛选检测,对判断HBV复制程度、病程、疗效及预后评估均有临床价值。

Clinical Analysis of HBcAg ELISA Result

Objective To study the positive rate of HBcAg,distribution pattern in HBV infected persons and its correlation to other hepatitis B marker,and to evaluate its clinical value in reflecting HBV replication,infectivity and therapeutic effect.Methods Six serum hepatitis B markers(anti-HBc,anti-HBe,HBsAg,HBeAg,anti-HBs) were detected by enzyme linked immunosorbent assay(ELISA) in 416 cases of HBV infected persons and nine combinations of various positive and negative markers were obtained for contrast analysis.Results HBcAg positive rate was 56.7% in 416 HBV infected persons,with none positive in 70 healthy controls and 65 patients with single positive anti-HBs.Conclusion Positive serum HBcAg by ELISA in HBV infected persons is of high specificity and the assay could be useful in those with positive HbeAg possibly reversed from positive anti-Hbe,which is valuable in determining their present HBV replication activity and infectivity and in assessing the therapeutic effect and prognosis.