高效液相色谱法测定注射用炎琥宁的含量

目的建立测定注射用炎琥宁的含量的高效液相色谱方法。方法 采用Diamonsil^TM C18柱（4．6mm×200mm，5μm），甲醇-0．05mol·L^-1。磷酸二氢钾溶液用磷酸调pH值至（2．5±0．05）]（68：32）为流动相，流速：1．0mL·min^-1，检测波长为251nm。结果炎琥宁在0．04312～0．1724mg·mL^-1峰面积与其测定浓度呈良好线性关系（r＝O．9999），平均回收率为99．9％，重复性试验RSD为0．49％（n＝6）。结论本法准确、简便、快速，可用于注射用炎琥宁的含量测定。

Determination of the content of potassium sodium dehydroandroan drographolide succinate for injection by HPLC

Objective To establish an HPLC method to determinate potassium sodium dehydroandrandrographolide succinate. Methods The separation was performed on DiamonsilTM C18 column （4.6 mm×200 mm, 5 μm）. The mobile phase was composed of methanol - 0.05 mol ~ L^-1 potassium dihydrogen phosphate solution -diethylamine pH adjusted to （2.5±0.05） by H3PO4] （68 ： 32） with the flow rate of 1.0 mL· min^-1 , and the detective wavelength was 251 nm. Results There was a good linearity within 0. 043 12～0. 172 4 mg ·mL^-1 of potassium sodium dehydroandran drographolide succinate （r=0. 999 9）. The average recovery was 99.96%, and the RSD was 0.49% （n=6）.Conclusion This method is accurate, simple and rapid. It is suitable for the quality control of potassium sodium de- hydroandrandrographolide succinate for injection.