巢式PCR检测先天性心脏病心脏石蜡标本中B19病毒的感染

目的：探讨微小病毒Ｂ１９与先天性心脏病（ＣＨＤ）的相关性及可能致畸机理。方法：采用病例对照研究，病例组为２９例ＣＨＤ尸解心脏组织，对照组为３０例同期非先天性畸形尸解心脏组织，应用巢式ＰＣＲ扩增Ｂ１９－ＤＮＡ，单纯疱疹病毒（ＨＳＶ），兔弓形虫（ＴＯＸ），巨细胞病毒（ＣＭＶ），结果：２９例ＣＨＤＢ１９－ＤＮＡ５例阳性，全瓿对照组为均阴性（Ｐ＝０．０２３７），ＨＳＶ，ＴＯＸ两组中均阴性，ＣＭＶ在两组中均

Investigation of parvovirus B19 infection in paraffin embedded cardiac tissues of congenital heart disease by nested PCR

Aim:To investigate the correlationship between the human parvovirus B19 (HPV B19) and congenital heart disease (CHD) and the teratogenic effect of HPV B19. Methods: A case control study was conducted to investigate the embedded autopsy cardiac tissuse of 29 cases of CHD and 30 cases of health controls with nested polymerase chain reaction (PCR) technique. Other virus, including herpes simplex virus (HSV), Toxoplasma gondii (TOX) and cytomegalo virus (CMV) were also detected in 10 cases of both groups respectively with corrospending PCR kit. Results: 5 of 29(17.24%) CHD cases were positive for HPV B19, while all the 30 cases of controls were negative for it ( P =0.0237), and all the detected cases were negative for both HSV and TOX. Three cases in each group were positive for CMV, with the presence of HPV B19 DNA in 2 cases of CHD groups. Conclusions: It is the first report of the presence of HPV B19 in cardiac tissues of CHD patient. The detection rate of HPV B19 DNA is significantly different between two groups, so it might be inferred that HPV B19 is correlated with CHD.